North Caucasian federal scientific center of horticulture, viticulture, winemaking
Author list of organization
List of articles written by the authors of the organization
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A MODIFIED PROTOCOL OF RNA ISOLATION FROM MATURE LEAVES OF GRAPES FOR RT-PCR
Description
Isolation of high-quality RNA from the tissues of perennial woody plants, including woody grape vines, is very difficult due to the high content of phenolic compounds, secondary metabolites and polysaccharides and the ribonuclease activity of destroyed tissues. Most of the existing methods require either large time or financial costs, or do not give reproducible results in the case of RNA extraction from mature grape tissues. The modified isolation protocol is based on a combination and modification of the known RNA extraction methods, taking into account the characteristics of mature grape tissues. Existing commercial kits for the isolation of RNA from plant tissues showed a low efficiency of RNA extraction from mature grape tissues, primarily associated with "varietal specificity". Reproducible results in the extraction of RNA showed CTAB-method, however, it has several significant drawbacks associated with the duration of the extraction and the complexity of the processing of an RNA preparation with a DNAase. The developed method is based on increasing the concentration of mercaptoethanol and polyvinylpyrrolidone in the extraction buffer, eliminating the stage of RNA selective precipitation via LiCl, and replacing it with deposition on a silica-based membrane (SiO2) followed by processing with DNA-ase. and increase the purity of the preparation of RNA from genomic DNA in comparison with the original method. A modified isolation protocol was developed based on a combination and modification of known RNA extraction methods, taking into account the characteristics of mature grape tissues. This solution allows to obtain reproducible quantity and quality of RNA for the subsequent synthesis of cDNA and RT-PCR
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05.20.01 Technologies and means of mechanization of agriculture (technical sciences)
Description
The urgent task facing the agro-industrial complex is the improvement of storage methods for agricultural products and the development of new ones. One of the actively cultivated crops, characterized by a variety of species, valuable taste and medicinal properties – grape. It is possible to provide the population with fresh grapes of high quality through the introduction of effective storage technologies that reduce losses, preserve marketable properties and biological value. One of the effective ways to preserve the quality of grapes during storage is the use of sulfur dioxide (SO2). This gas inhibits oxidative enzymes in berries, thereby reducing the rate of development of the phytopathogen Botrýtis cinérea, which causes gray rot. At the same time, it is especially important to control the rate of release of sulfur dioxide, since at the beginning of storage it is necessary to ensure the receipt of a large amount of it, which will eliminate Botrytis spores present on the surface of the berries and stabilize the existing damage. Further, during the entire period of storage of grapes, sulfur dioxide must be supplied in minimal quantities. Such dynamics of SO2 emission can be ensured by the use of two-phase generators of sulfur dioxide. The article investigated the effects of sulfur dioxide generators on the quality of grapes during storage. The study also investigates quality indicators of grapes of several varieties zoned in the Krasnodar region under long-term storage
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05.20.01 Technologies and means of mechanization of agriculture (technical sciences)
Description
The article presents the results of studying the influence of some types of packaging materials used in conjunction with Inter Fresh’s ethylene absorber (sachets) on commercial quality and losses of Alexandria zucchini, Lyubov sweet pepper and tomatoes cv. Inkas during storage. The study was carried out using StePac Xtend bags, creating a modified atmosphere. The objects of research were stored for 14 days at a temperature of 8 – 10 °С and air relative humidity of 90±3 %. One sachet for ethylene absorption was placed in Xtend bags. It was found that when stored under these conditions, the total weight loss is: for tomatoes – 0.3% when using Xtend bags and Inter Fresh ethylene absorbers, 1.8% when using traditional packaging materials; for zucchini – 0.4% when using Xtend bags and Inter Fresh ethylene absorbers, 1.6% when using traditional packaging materials. Losses associated with microbiological damage during storage of zucchini and tomatoes were not observed. During storage of sweet pepper microbiological spoilage took place, which, apparently, was the result of increased contamination with pathogenic microorganisms
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APPLE ROSETTE DISEASE IN NORTH CAUCASUS PLANTATIONS
06.01.01 General agriculture, crop production (agricultural sciences)
Description
The article presents the results of a study of the apple tree rosette disease found in a collection garden and having a viral nature. The rosetting has typical signs of manifestation. Sick plants develop narrow small chlorotic leaves. Rosettes of leaves on the upper cervical and lateral shoots are formed as a result of the convergence of internodes. The spread of the rosetting in the planting reaches 10-11 %. The prevalence of viral disease varies among the 44 varieties of apple trees of different ecological and geographical origin. So, the lesion of rosetting was noted on single trees in the varieties of Gala Redi Red, Fujiko, Red Free, etc., and it was a complete defeat in the variety of Piros. A different degree of the manifestation of the disease on the trees was established: from partial manifestation on individual shoots, to aggressive damage to the entire crown of the tree. On young trees bearing fruiting (3-4 years from planting), the rosetting reduces the yield of apple trees by 36 %, ¼ part of the fruits on the trees are small in size compared to the fruits of healthy trees. The infectious nature of the rosetting is confirmed by the transfer of the disease on the most affected Piros variety using summer budding
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