03.00.00 Biological sciences
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CYCLICAL EFFECTS OF ACTINOMYCIN D ON THE BARLEY COLEOPTILES GROWTH
DescriptionIn cell biology, actinomycin D is shown to have the ability to inhibit transcription. Actinomycin D does this by binding DNA at the transcription initiation complex and preventing elongation of RNA chain by RNA polymerase. When soaking the seeds in a solution of actinomycin D, antibiotic blocks RNA synthesis and seed germination occurs at the expense of long-living RNA, available in the mature seed. In the article we present experimental data indicating that as storage seeds of winter barley are changing the action actinomycin D on the growth of coleoptiles. It is shown that actinomycin D at a concentration of 40 µg/ml in October reduced the growth, in December it was not authentically, and in February it was paradoxical amplified growing coleoptiles. We suggest the part of the actinomycin D fastened with growth inhibitor coleoptiles, whose structure changes during seed storage and increase the affinity of actinomicyn D to the inhibitors. At a concentration of 60 µg/ml actinomycin D effectively reduced of the coleoptiles growth. Suppression of seedlings roots growth was proportional to the concentration of the actinomycin D and exposure time
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ECOLOGICAL BASES OF SIMBIOGENIC DEVELOPMENT OF PLANT AT COMPLEX SOWINGS
DescriptionDevelopment of plants in mixed or combined sowings is based on the symbiotic association in the basis of which we have decreased sexual process and its mechanism of genetic exchange among endosymbionts and stimulating of genetic exchange and expansion of the diversity of species among exosymbionts. The exception is the symbiosis of orchids and fungi - activators of orchids mycorrhiza with developed micellar system easily passing on saprophytic option. Coevolution of plant organisms with fungi indicates the specifics symbiogenic conjugate evolution, showing an example of the evolution of a small community with different contact with the external environment. The phenomenon of symbiogenesis has a long history and was manifested in a variety of different types of organisms. Today, some dependence of existence of one species from other organisms is observed at approximately 75% of higher plants and 90% of the animals in varying degrees of symbiogenic dependence from other species and taxa. Originality of symbiogenesis was analyzed by Academician A.A. Takhtadzhyan (1973) during its consideration of origin of eukaryotic cells as process of "assembly" of a complex system. Different approaches to determining the biological essence of symbiosis are known in the history of study of this phenomenon. On the whole phenomenon of symbiosis belongs to the category mutual relations of organisms of different phylogenetic groups
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Description
In herbaceous communities of annual crops dominate clean sowings, which are characterized by high productivity. However, it is very poorly adapted, different weak transformation of matter and energy and stronger exposed to stressful situations as compared to natural systems. In agroecosystems only structural diversity can supporting many processes on a much aligned level. In agrolandscape system creates mixed sowings that are practiced in forage production of many areas. Great importance is the selection of crops for joint sowing because the relationship of species in created systems are poorly investigated and documented in the literature is not enough. Investigations were carried out on experimental plots on the farm called "Zavety Ilyicha" of the Leningrad district and training farm called "Kuban" in Krasnodar. Formation of joint sowings in different moistening conditions, level of fertility, chemical and physical condition of the soil is a very big problem. Cultivation of different cultures in joint sowings significantly influences the terms the onset of main phases of vegetation in certain species. For example, the placement of sorghum between rows of soybean noticeably extended the period of its vegetation (5-7 days); acceleration of interphase periods was marked for amaranth; height of plant noticeably was changed, leaf area was varied, indicator of competitive features of individual species was differed. The existence of the relationship between the method of sowing crops, their farming practices and composition of species and population of microflora, mesofauna of soil, yield of dry matter and grain was observed
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PERSPECTIVES OF DEVELOPMENT OF AGROLANDSCAPES IN THE STEPPE ZONE OF EDGE
DescriptionStudies of ecosystems allow estimation of difficult processes (physical, chemical and biological), taking place in them and determine how to manage them. At the heart of the natural systems there are freely interacting populations of different taxons, productivity of their above-ground structures, details of which are quite limited, and as underground systems, the data have even less information. In the late 60s and early 70s in the XX century a lot of information appeared associated with problems of environment, its pollution, lack of energy of food resources, clean water; it determined the root cause of the reaction of the population of developed countries on the aggravation of relations between society of people and habitat. Existed for a long time the concept of the exploitation of nature, which reflected on using soils, forests, water and other sources and which showed the power of mankind and its technical power over nature. In reality, this concept was just a common strategy of aphids sucking their environment. Understanding the real situation on the different levels of consciousness of the population defined situation of practical and scientific research in the field of ecology of different directions - chemical, biological, mathematical and other sciences. Development of ecology today will evolve to meet conservation and sustainable development of the biosphere, which experiencing constant anthropogenic pressure. An important problem of agrolandscape systems is an objective assessment of their productivity. Among the issues that determine the development of agricultural systems there are the natural conditions and soil fertility, crop dynamics over the years, the duration of the growing season of individual taxons, as well as the possibility of create of mixed crops, different type of life form, ecological features, physiological diversity of biochemical characteristics. Sowings this type allow, under certain conditions, obtaining stable yields under any climate dynamics and significant reduction of soil erosion. Combined sowing that is practiced in South East Asia, particularly in China, where the main components are the soybean and corn. The usefulness of mixed crops is the stability of yields over the years and the ability to emphasize their resistance to dry weather conditions
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THE METHODS OF REPRODACTION OF LAVANDULA ANGUSTIFOLIA MILL.
DescriptionThe article presents results of the study of lavender reproduction (Lavandula angustifolia Mill) with methods of generative reproduction (seeds), vegetative reproduction (cutting, layening, divide of buch), clonal microreproduction using the culture in vitro. The method of cloned microreproduction based on the culture of the isolated meristems which provides genetic identity of regenerated plants to initial forms and high coefficients of reproduction, improvement of landing material from fungal and bacterial infection, and also release from viruses at a combination with methods of thermotherapy and a chemotherapy is the most effective for the solution of objectives of seed farming of a lavender. As a material for carrying out researches we had plants of lavender (Lavandula angustifolia Mill), Sineva sorts, Stepnaya and perspective selection samples 337-9 and 310-17. Apical meristems 0,2-1,0 mm high isolated from top and axilary buds of a stalk of annual plants have been used as explant. Murasige and Skuga (MS) used as a basic nutrient medium for cultivation of the isolated meristems. Explants have been cultivated in the cultural room with 25-26 0C - temperature, lighting – 2-3 lx, relative humidity – 60-70%. It has been established that both April and October are the best month for isolation of meristems correspond calendar to phases of spring and autumn growth at the lavender donor plants and that optimum is an agar nutrient medium of MS, added with kinetin (1,0 mg/l) and GC (1,0 mg/l); frequency of regeneration of all studied genotypes was 90,0-100,0 %. Feature of morphogenesis of lavender meristems in vitro culture was already at the first stage of clonal microreproduction as there was a multiple shoot formation. Studying of features of development of lavender microplants during ten passages also was carried out, as the level of stability of regeneration processes throughout several cycles of a miсrograftage is one of important factors on which efficiency of microreproduction depends. We have also shown, that the efficiency of reproduction remains at the stable level of the sort named Sineva and sample #337-9 to the 8th passage (1:7,77-12,45 and 1:7,60-11,85 respectively), at the variety called Stepnaya - to the 7th passage (1:6,10-11,81), at the sample #310-17 - to the 6th passage (1:6,17-8,37)
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Description
This article is devoted to treatment effects of cuttings of a grape variety called Moldova with 0.01% solution of heteroauxin on their regenerative capacity, depending on the light conditions. We have established that the germination of cuttings in the dark, as well as the treatment of heteroauxin significantly stimulate their regenerative abilities. The length of the shoots, regardless of the use of heteroauxin, depends on the mode of the light produced during the germination of cuttings. In both studies, the length of the shoots on the end of experiment cuttings germinated in the dark, was 1,71-2,66 times more than when germinated in the light. Cuttings rooting ability also largely depends on the light produced in their germination. In both studies, the rooting of cuttings started earlier and passed more rapidly during their germination in the dark. Processing cuttings with heteroauxin, followed by germination in the dark, speeded up the formation of roots even more, compared with the case where the cuttings treated with heteroauxin germinated in the light. In the best case rooting increased by 42,5-47,5%, the number of roots on basal ends of cuttings in 18,3- 18,5 times, the numbers of cuttings with 3 or more roots to 72,5-80,0%
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BIO-DIAGNOSTICS OF RESISTANCE OF GREY FOREST SOILS OF ADYGEA TO POLLUTION WITH Zn, Cd, Mo, Se
DescriptionThe essential part of a soil cover of the Republic of Adygea is occupied by gray forest soils. Thus they still remain a little studied, including concerning their resistance to chemical pollution. Contamination of gray forest soils of Adygea with Zn, Cd, Mo, Se causes deterioration of their biological properties. In most cases, the degree of reduction of the values of biological indicators is directly dependent on the concentration of pollutant in the soil. According to the degree of toxicity to the biological properties of the investigated elements form the following sequence: Se > Zn > = Cd > Mo. Biological parameters investigated in research (activity of catalase and dehydrogenase, cellulolytic ability, abundance of bacteria of the genus Azotobacter, radish root length) may be used for purposes of monitoring, diagnosis and regulation of chemical pollution of gray forest soils Zn, Cd, Mo, Se
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Description
This article is devoted to the study of small mammal communities (species composition, daily activity and spatial distribution) in the agrocoenosis. The fields of winter crops that were surrounded by similar fields or by winter crops with meadow vegetation were researched. The Mus musculus became the dominant species in the winter crops agrocoenosis. The R. Sylvaemus living near the border with field-protective forest belts (and 25 meters field around) are subdominant group. Marked single finds Mus musculus in the forest and R. Sylvaemus in the field (at a distance before 50 meters). It is shown that the presence of meadow vegetation increases the species diversity of mice in the agrocoenosis. In the middle of this field a subdominantion group is Microtus arvalis s.l. and Cricetulus migratorius. The both species prefer a neighborhood with meadow vegetation. The method of recapture, followed by labeling with animals, made it possible to fix the movement for a distance of about 400 m. during the night (maximum values were males Mus musculus). The activity of small mammals in the winter crops was timed to coincide with the day-night cycles, with high activity from 01:00 to 04:00 am, and minimum activity after 09:00 hours of the morning before 08:00 pm
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Description
The results of the study presented showed that predator mite Ph. persimilis exhibited high food preference of the larval stage (30,2% of the total number consumed) compared to the egg (20,5%), nymph (13,4%) and adult (10,1%) stages of two spotted spider mite T. urticae. The functional response of Ph. persimilis feeding on eggs and adults of T. urticae was described by the Type II curve. At a maximum density of 60 prey per leaf disc, Ph. persimilis consumed an average of 22.8 eggs and 3.2 adults over an eight-hour period. Calculated time for handling of the prey by Ph. persimilis was on the average 0.074 hours (4.4 minutes) and 3.2 hours for T. urticae eggs and adults respectively. The mean number of T. urticae eggs consumed by Ph. persimilis progressively increased with the increase of the prey density, but the rate of predation (number of eggs consumed/prey density) decreased from 0.5 to 0.3 with the increasing of prey density. The mean number of adult T. urticae consumed by Ph. persimilis at densities of 20, 40 and 60 per leaf disk remained relatively constant. But the rate of predation decreased from 0.09 to 0.04 with increasing of prey density. The experiments showed that regardless of the strategy utilized by the predator it was the one that was efficient for the predator survival
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CHARACTERISTICS OF THE DYURSO RIVER ICHTHYOCENOSIS
DescriptionIn the article we have described the history of the studies and the modern features of fish community of the Black sea's river Durso (the length 14 km). It was established, that in present time the river includes only seven species of fishes from three ecological groups: freshwater (five species), saltwater (one specie) and marine (one specie). A bleack (Alburnus alburnus) and Colchis minnows (Phoxinus phoxinus colchicus) dominate by quantity in the structure of Durso river fish community, bleack and Prussian carp (Carassius auratus gibelio) dominate by biomass. The negative changes of Durso river conditions were found in compare with the last years. First of all, it appears in biological pollution of fish species allochthonous for this river. In the period of the Durso river monitoring (1935–2014) it was established the distinct raise of allochthonous components with parallel reduce of variety of autochthonous fresh waters rheophilic species. The main reason is the building of reservoir in upper flow of the Durso river in 1976. The additional negative factor influence on of the Durso river fish community is the falling down of the water level during the important period of fish lifecycle. The best condition of fish community is in the middle part of the river were reaches combine with the cold springs and powerful flow