03.00.00 Biological sciences
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BIOLOGICAL VALUE OF PLANT PROTEIN AND VITAMIN SUPPLEMENTS
DescriptionResults of the use of plant protein feed additives containing pumpkin paste and soybean seeds of different varieties for quail are presented in the article. It was established that the use of such additives on the basis of Valens soybean allowed to receive higher growth parameters than groups treated with Vilan soybean additive
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BIOLOGICAL CHARACTERISTICS AND COMMERCIAL STOCKS OF HUMPBACK WHITEFISH IN YAKUTIA WATERS
DescriptionHumpback whitefish - Coregonus lavaretus pidschian (Gmelin, 1788), is spread in Siberia, in the Kara, Laptev and East Siberian seas and in the lakes of the Arctic and Subarctic. Objective: To assess the status of fish in the waters of Yakutia, to make ecological forecasts and determine the possibility and prospect of economic use of whitefish, as a fish-breeding material in the present conditions. Methods: The analysis was carried out on the fresh material as described I.Pravdina (1966), taking into account the recommendations of Y. Reshetnikov (1980). Collection and processing of materials were carried out in 1969-2015 by standard methods. Results. Reaching sexual maturity at the sixth (in weight - the eighth and ninth) year of life, whitefish begin to move to the main breeding groundsin the early fall. Absolute fecundity of eggs 2500-140000. Spawning is common in late September-early October. The food spectrum usually consists of the following objects: chironomid larvae, plants, mollusks, fish eggs and copepods. By the nature of power - benthophage, but often becomes planktonic organisms. Conclusions. Important food fish, but its reserves significantly reducedunder the influence of anthropogenic factors in recent years. The catch of whitefish can be increased as a result of the development of aquaculture
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BIOLOGICAL RECULTIVATION OF OVERBURDEN ROCKS IN KURSK MAGNATIC ANOMALY (KMA) REGION
DescriptionThe results of biological recultivation of disturbed lands are offered in the article. Silvicultural and taxation parameters of basic wood types of dumps and their role in stable ecosystem formation are presented
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BIOLOGICAL ACTIVITY OF URBAN SOILS (ON THE EXAMPLE OF ROSTOV-ON-DON)
DescriptionAbout 40 soil cuts were made in various parts of the city of Rostov-on-Don. The determinations of enzyme activity (catalase, invertase, polyphenoloxidase) were carried out in natural and antropogenic soils of a sub-urban park, residential and industrial areas of the city. The enzyme activity of soils in urban condition signif-icantly lowers than in non-urban soils. It is established, that significant impact on the biological activity has duration of sealing, and the longer the period, the higher the negative impact on the enzymes activity. However, a close relationship between humus state of soil and its enzyme activity kept in a condition of ur-ban pedogenesis. The high activity of investigated enzymes is typical to soil horizons which contain the highest amount of soil organic matter
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BIO-DIAGNOSTICS OF RESISTANCE OF GREY FOREST SOILS OF ADYGEA TO POLLUTION WITH Zn, Cd, Mo, Se
DescriptionThe essential part of a soil cover of the Republic of Adygea is occupied by gray forest soils. Thus they still remain a little studied, including concerning their resistance to chemical pollution. Contamination of gray forest soils of Adygea with Zn, Cd, Mo, Se causes deterioration of their biological properties. In most cases, the degree of reduction of the values of biological indicators is directly dependent on the concentration of pollutant in the soil. According to the degree of toxicity to the biological properties of the investigated elements form the following sequence: Se > Zn > = Cd > Mo. Biological parameters investigated in research (activity of catalase and dehydrogenase, cellulolytic ability, abundance of bacteria of the genus Azotobacter, radish root length) may be used for purposes of monitoring, diagnosis and regulation of chemical pollution of gray forest soils Zn, Cd, Mo, Se
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Description
In this article, we have presented a number of modeling experiments to investigate the chemical pollution of brown calcareous soil. It assesses the stability of brown calcareous soil of a nature reserve called Utrish to contamination with heavy metals in biological parameters. Pollution of brown calcareous soil with Cr, Cu, Ni, Pb and oil causes a significant reduction in biological parameters. The degree of reduction depends on the nature of the contaminant and its concentration in the soil. The study showed that the indicators used in the biological condition of the soil, could be recommended for use for diagnosis, monitoring, and regulation of chemical pollution of brown soil. According to the degree of resistance to chemical contamination, the brown soils of Russia form the following series: typical brown - brown carbonate - brown leached
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PROTEINS OF AFRICAN SWINE FEVER VIRUS
DescriptionThe review presents some data on structural and non-structural, regulatory proteins and enzymes of African swine fever (ASF) virus. The variety of the virus biological characteristics is substantially caused by proteins belonging to multigenic families. It is suggested, that the protection development at ASF is provided not only by the membrane proteins, but also by the regulatory ones
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APPROBATION OF MULTIPLEX SSRANALYSIS FOR DNA-FINGERPRINTS OF RICE VARIETIES
DescriptionResults of testing of multiplex sets SSR-markers for genotyping of rice varieties are presented in the article. Two sets of SSR-markers were formed: 1: RM1+ RM11+ RM70+RM122; 2: RM164+RM167+RM168. The optimal combination of DNA markers in the multiplex sets and PCR conditions allowed obtaining accurate, easily interpretable results when performing fragment analysis on automated genetic analyzer ABIprism3130. Using multiplex sets, genotyping was performed for several varieties of rice: domestic breeding and one variety – IR36 from the breeding of IRRI (Manila, Philippines). For all the studied varieties specific SSR-fingerprints were obtained. RM 168 marker showed in domestic varieties a low level of polymorphism - one allele of 97 bp. However, at the same time, the variety IR-36, showed a second type of allele 107 bp. In addition, the loci of RM1, RM11, RM167 and RM164 have unique alleles in this variety. It is consistent with significant genetic differences of these varieties and the rest of the varieties in studied sample. The proposed SSR multiplexes are promising for use in DNA certification of rice varieties and assessment of genetic diversity
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APPROBATION OF MICROSATELLITE DNA-MARKERS FOR IDENTIFICATION OF APPLE CLONES
DescriptionAs the result of the work, the test of microsatellite DNA markers for the identification of genomic polymorphism between apple cultivars Florina, Golden Delicious and clonal forms has been done. With the high level of intervarietal polymorphism, studied DNA markers showed no allelic differences between varieties and their clonal forms
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Description
The study was performed to genotype some commercial wine yeast strains using the assay of Interdelta genomic sequences. Experimental parameters of PCR to identify were optimized and optimal simplified method of DNA extraction from dried preparations of yeast cultures was define. Proven method showed a high level of resolution and can be used for the analysis of genetic diversity wine yeast in combination with SSR-markers