16.00.00 Veterinary sciences
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MONITORING OF THE CANICOLA FEVER IN POPULATION OF CATTLE AND WILD FAUNA OF THE AMUR REGION
DescriptionThe article presents results of researches of authors on prevalence of a canicola fever in populations of cattle and wild rodents in the Amur region. The research objective consisted in studying of etiological structure of a canicola fever of cattle and establishment of the dominating canicola fever originator serotypes at the rodents living in the Amur region. Results of researches showed that in etiological structure of a canicola fever of cattle in the Amur region the admixed serogroups and a serogroup of Sejroe dominate. The natural reservoir of a canicola fever in the Amur region are populations of gophers, chipmunks, muskrats and voles. The highest percent of the infected individuals is taped in population of gophers (57,1 %). Level of contamination of animals in populations of muskrats and chipmunks made 20 and 17,6 % respectively. Contamination the canicola fever originator in populations of voles was at the level of 8-9 %
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Description
The level of development of modern medical equipment for the past 20-30 years is in constant development, many of the technologies and methods of treatment of certain diseases that were previously only available for the treatment of a person with success began to penetrate in the veterinary practice. The objective aim at the initial stage was the establishment of testing and suitability for the cultivation of autologous dermal fibroblasts animals of previously known techniques and the modernization of the existing techniques. Next, we had to carry out measurements of morphometric parameters and to identify structural features and functional activity of fibroblasts of different kinds of agricultural animals. Conclusions: The study showed the conducted morphometric autologous dermal fibroblasts tests allowed us to obtain the correlation line. In addition, to identify common patterns in the development and growth of fibroblasts derived from different species of agricultural animals. And also, to obtain material for comparative evaluation of the quality of the obtained cell cultures using entropy equivalent
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MORPHO-FUNCTIONAL STATE OF BROILER CHICKENS ADRENAL CAPSULES UNDER DIFFERENT METHODS OF MANAGEMENT
DescriptionAn impact analysis of abiotic factors of ecosystems on the metabolic processes of the broiler chicken organism under various methods of poultry operation has been carried out. The analysis results confirm that the enorganic biochemical processes are under the influence of the environment, and any changes in its condition cause the development of response reactions of the organism at the biochemical level. The source of control of the relative constancy of the milieu interieur is the interaction of tropic and effector hormones. Adrenocorticotropic hormone and cortisol are the main hormones of adaptation. The activity of the adrenal capsules turns to be an effector peripheral link of the reflex chain of the adaptive reaction of the organism. Adrenal hormones have a direct impact on tissue metabolism. The following aspects have been taken into consideration: technological growing conditions, production parameters, morphological blood values of chickens of different ages, quantitative content of adrenocorticotropic hormone and corticoid in the blood, morphology of the adrenal capsules. The abiotic factors of the cage management system contribute to an increase in the concentration of corticoid in the blood of broiler chickens, compared with this value of the broilers grown under the floor management
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SOME IMMUNOBIOLOGICAL FEATURES OF RIFT VALLEY FEVER VIRUS OF ATTENUATED STRAIN
DescriptionThe article represents some results of Rift Valley virus culture characteristics, development of conditions for virus inactivation with teotropin, formalin or ethyleneimine dimer. A range of inactivants and optimal inactivation conditions, which provide the production of inactivated raw virus with preserved antigenic and immunogenic activity rates, is selected
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DETERMINATION OF ACUTE TOXICITY OF ROKSATSIN
DescriptionIn this article, the authors have defined the acute toxicity of Roksatsin preparation, which represents a 20 % aqueous solution of polyhexamethylene guanidinehydrochloride (PHMG). The acute toxicity of Roksatsin in the first series of experiments was studied on white mice upon intragastric administration. In the second series of the experiments, Roksatsin was administered subcutaneously to white rats and in the third series of experiments the preparation was administered intracisternally to cows. The results of the first series of experiments showed that Roksatsin by the internal method of appointment is slightly toxic to white mice. In this experiment not been a single case of acute intoxication and death of animals. Experiments to determine the acute toxicity of the preparation for white rats were performed in triplicate. In the first series of experiment, the LD50 was 4.8 ml/kg. It is found, that the calculated coefficients LD50 for other two series were close to the first one: the second - 4.2 ml/kg and the third - 4.5 ml/kg. Therefore, the authors found that the Roksatsin preparation upon subcutaneously administration according GOST 12.1.007-76 is a low-toxic compound (4th class of danger). As a result of the third series of the experiments, it was found that the intracisternally administration of Roksatsin preparation to cows in a dose of 5 ml is not toxic to the animal organism. Intracisternal administration of Roksatsin does not have a significant impact on the morphological and biochemical indices of blood. On this basis, Roksatsin preparation with different routes of administration is low-toxic and it can be recommended for clinical researches
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Description
The diagnostic preparations created on the basis of polymeric carriers, find wide application in diagnostics of various diseases with the purpose of detection of small quantity of antibodies or antigenes in various substrata. Due to the physical and chemical properties, polymeric particles are convenient carriers at creation of various diagnostic tests - systems in area immunoassay. It is possible to attribute the following moments to advantages of reaction of latex - agglutination: simplicity and speed of performance, absence of necessity for the complex equipment, reproducibility and accuracy, an opportunity of reception of the big parties of standard and homogeneous polymeric suspensions
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EXPERIENCE OF USING NATURAL MINERAL COMPOUNDS FOR METABOLIC DISORDERS IN CATTLE
DescriptionIn this study we show the results of the use of natural mineral compounds in metabolic disorders in cattle-breeding complexes of industrial type. According to official data for 2013-2015, based on the biochemical analysis of blood of cattle, that was held in some farms of Samara region, significant metabolic disorders were determined due to decrease in total protein level on 31,5% in average, decrease in alkali reserve – on 26,9% of samples, decrease in calcium concentration – on 40,7%, decrease of the phosphorus – on 8,1%, decrease in carotene – on 81,7% and glucose – on 84,3% of studies. Clinical examination of herd revealed a high level of animals with obvious signs of mineral metabolism disorders (osteodystrophy and rickets of alimentary origin) and comorbidity (nutritional anemia, gastroenteritis, pneumonia, disturbance of protein, carbohydrate and vitamin metabolism, and others). The use of complex natural compounds improves the efficiency of the traditional complex of treatment and prophylaxis, causing an increase in the total concentration of calcium in the blood serum on 10-55%, increase in calcium-phosphorus ratio till 1,27-2,3, increase in annual growth rate of young animals’ body weight on 8,2-44,8%, increase in milk production on 5,9-16,3%. Preparations have an antianemic action, normalize indicators of micronutrient metabolism of proteins and carbohydrates, enhance non-specific resistance factors and prevent the development of non-contagious internal pathology
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CHARACTERISTICS OF A HUMORAL IMMUNE RESPONSE OF STURGEONS TO A HERPES VIRAL DISEASE AGENT
DescriptionThe article presents some results of the studying of the characteristics of the course of herpes viral disease in various sturgeon species and their hybrids, and the dynamics of their specific humoral response to this infection
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PECULIARITIES OF ERYTHROCYTE ATPASE FUNCTIONING OF CHICKEN-BROILERS
DescriptionPeculiarities of functioning of ATPase of erythrocyte of chicken-broilers are considered in the article. Method of discharge of cytoplasmic membranes and nuclei of erythrocytes of chicken-broilers was proposed. Data on influence of ions Na+ and K+ on activity of general ATPase, localized in cytoplasmic and nuclear membranes were presented.
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Description
According to the veterinary reporting on 01.01.2016, presence of a bird in the agricultural organizations and poultry farms makes 13 million 440 thousand heads, in the private sector (hens, geese, ducks, etc.) - 6 million 287 thousand heads. In 2015 in the Krasnodar region 3 million 337 thousand 127 heads were subjected to bacterination against smallpox and a diphtheria. Data on registration of outbreak of smallpox of birds in a private farmstead of the Krasnodar region are presented in article. The case rate and a mortality at hens of breeds of Kulanga and Bramah and pigeons of the Andijan breed are studied. The dermal, diphtheroid and admixed forms of clinical implication of smallpox at hens of different breeds and pigeons in this farmstead are studied. Pathoanathomical features of implication of smallpox of birds are studied. The biological test on chickens of 3-4-month age and a microscopy of the painted smears prints on Pashena is carried out. In smears Pashen's little bodies are found. The events for elimination of a disease in this LTD including rejection of a sick bird, the compelled bacterination at an acquisitive bird, mechanical cleaning and disinfection in a hen house and a dovecot, isolation and a symptomatic treatment of breeding birds are developed and held