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06.00.00 Agricultural sciences
DescriptionNew varieties of winter durum wheat selected by methods of complex crossing varieties of winter durum wheat of different ecological-geography region and their mutants differ with quality of grain adaptability to econiche. Value of chemical mutants in selection of winter durum wheat on adaptability and quality of grain is discussed
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Description
Analysis of 40 years’ making and evolution of one of the subject area on Electric machines and electric drive department is presented
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Description
Influence of circuit and constructive signs of stator and rotor windings on parameters and operational properties of asynchronous generators is examined
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Description
Influence of circuit and constructive signs of stator and rotor windings on parameters and operational properties of asynchronous generators is examined
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EXTRACTION OF DNA FROM HERBARIUM LEAVES VITIS VINIFERA L.
06.00.00 Agricultural sciences
DescriptionTwo methods were used for DNA extraction from herbarium leaves of two subspecies: V. vinifera subsp. sativa D.C. и Vitis vinifera ssp. silvestris Gmel. DNA extraction is difficult from herbarium leaves plants because of the presence of metabolites that interfere with DNA isolation procedures and downstream applications such as DNA restriction, amplification, and cloning. The quality of DNA was checked on a spectrophotometer by measuring the level of absorption at A260/280 nm. The results showed the successful extraction from mature and dried leaves of DNA when using the СТАB-method №2. Another method hasn’t shown satisfactory results in amplification of DNA
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EXTRACTION OF DNA FROM LEAVES OF VITIS VINIFERA L.
06.00.00 Agricultural sciences
DescriptionExtraction of the DNA from leaves of genus Vitis is a very difficult task, as a grape has a lot of elements, which make difficult to use it in the genetic manipulation. In that article used the methods of extraction of the DNA with silica (SiO2) particles as the absorbent material for getting better quality DNA
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06.00.00 Agricultural sciences
DescriptionUsing two microsatellite loci (HaSSR1, HaSSR3), the PCR analysis of the bollworm Krasnodar population was conducted. Molecular-genetic analysis (SSR-PCR) of different geographic codling moth populations by three microsatellite loci Ср1.63; Ср2.39 и Ср2.157 was conducted also. Based on the assessment of the frequencies of occurrence of DNA markers, the molecular-genetic structure was described and genetic diversity in the pest populations was estimated
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THE RESEARCH OF HAPLOTYPE DEVERSITY OF RUSSIA ABORIGINAL GRAPE VARIETIES
06.00.00 Agricultural sciences
DescriptionIn the article we have studied 55 aboriginal varieties, collected in the North Caucasus. We have described allelic diversity and identified four haplotypes. All the populations were closely related while being examined using a factor of Nei's genetic distance (Dn), with the exception of indigenous varieties of Armenia. Maximum differences have been found between the Uzbek population and Astrakhan, Turkmenian, Armenian populations. The most genetically rich were the population of Don area and Krasnodar region
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THE RESEARCH OF VITIS SILVESTRIS GMEL. IN THE NOTHERN CAUCASUS
06.00.00 Agricultural sciences
DescriptionVitis vinifera subsp. silvestris Gmel. is the wild subspecies of Vitis vinifera L. and an important source of genetic resources for grapes breeding. Currently, this form is very rare in the world. In this paper, conducted the first study of wild grapes which was found in the North Caucasus with the use of two microsatellite markers. The study have shown a high level of genetic diversity: intrapopulation variability was 75,2%, the interpopulation variability accounts was 24,8%. All populations are characterized by certain unique genetic structure and may be as donor of resistance genes which have a long-term evolutionary selection
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CREATION OF THE INDICATIVE AND GENETIC COLLECTION OF THE LARGE-MASS GRAIN OF THE GRAIN SORGHUM
06.00.00 Agricultural sciences
DescriptionThe inheritance of the “mass of 1000 grains” sign in the hybrids F1 and F2 was studied. Differences in the parental forms with 1-5 pairs of genes are revealed. The sources and the donors of the large-mass of grain are pointed, the indicative and genetic collections of the large-mass of grain forms of the grain sorghum are created