03.00.00 Biological sciences
-
HORSERADISH PEROXIDASE IMMOBILIZATION IN CALCIUM ALGINATE HYDROGEL AND MICROPARTICLES
DescriptionIn the article two differently obtained preparations of immobilized horseradish peroxidase are compared with free enzyme and with each other by their stability. It is shown that both preparations of immobilized peroxidase possess increased stability during storage and heating. It is established that activity of hydrogel obtained increases after heating
-
Description
The article gives the description and the algorithm of the simulation model SMDS "Potok" 1.0, designed to calculate the parameters of distribution and sedimentation of technological alluviums in watercourses. The relevance of the simulation model is due to the need to determine the integral indicators (the volume of water flowing through the areas of the plume with a predetermined suspension concentration, the average layer of the silt, the specific density of the bottom silt in the calculated zone) when calculating the harm to water biological resources from the implementation of various economic activities in accordance with the methodology of calculation of damage caused to water biological resources approved by the order of the Federal Agency for Fisheries No. 1166 of 25.11.2011. Usage of the simulation model of SMDS "Potok" 1.0 will increase the reliability of calculations in determining the damage to water biological resources, as well as bring the calculations of parameters of distribution and sedimentation of technological alluviums in watercourses in accordance with the current regulatory legal acts of the Federal Agency for Fisheries
-
Description
Artificial reduction of photoperiod among di-and-tetroploid stevia varieties (Stevia rebaudiana Bertoni) to 12 hours in the second part of the II stage organogenesis fastens the transfer to III stage organogenesis and induces blossoming for 30-32 days earlier than at natural photoperiod. This provides stable seed productivity under field tests. Blossoming features providing crossbreeding are discussed
-
ИНИЦИАЦИЯ ПРОЭМБРИОГЕННЫХ КЛЕТОЧНЫХ СУСПЕНЗИЙ У ДЕВЯТИ МЕЖВИДОВЫХ ГИБРИДОВ ВИНОГРАДA
DescriptionЧерешки листьев, взятых из растений ин витро, девяти межвидовых гибридов винограда: подвоев Берландиери х Рипариа ‘Кобер 5ББ’, Рипариа х Рупестрис ‘101-14’ и сортов (cvs): 'Бианка', 'Зигфридребе', 'Подарок Магарача’, ‘Первенец Магарача’, ‘Цитронный Магарача’, ‘Интервитис Магарача’ и гибридной формы ‘Магарач 100-74-1-5' культивировали на твердой среде NN, содержащей различные концентрации 2,4-D и BА в различных сочетаниях. Для того, чтобы инициировать рост клеточных суспензий, проэмбриогенные каллусы пересаживали в жидкую среду NN, дополненную 1.0 мг/л 2,4-Д и 0,2 мг/л BА. Субкультивирование этих суспензий в жидкую среду NN, дополненную 2 мг/л NAA и 0,1 мг/л BА, привело к развитию агрегатов эмбриоидов, а пересадка в жидкую среду с 0,5 мг/л BА вызвала развитие единичных глобулярных и сердцевидных эмбриоидов. Проэмбриогенные клеточные суспензии, состоящие из преимущественно отдельных клеток, могут быть использованы в трансформации генов и селекции на клеточном уровне с целью уменьшения вероятности появления химерных растений
-
Description
At present, the cultivation of agricultural products is on a level where the growth of productivity and quality is only possible by using the latest achievements of science. Scientists have been developing elements of agro-technologies of complex applications of growth regulators, fungicides and fertilizers in growing winter wheat, providing a significant increase in productivity and quality of agricultural products, reduction of labor costs, energy and all kinds of resources, sustainable harvesting, even in the zone of risky agriculture. Plant growth regulators have multifunctional properties, which are expressed in the regulation of plant growth and development, and in increasing their resilience to adverse weather conditions and many diseases. However, despite the fact that there are many examples of extremely high economic efficiency of plant growth regulators, in terms of production and use of pesticides are much inferior. Retardants and defoliants are used more widely. However, low rates of regulators and elicitors, the ability to manage with their help the growth and development of plants; change the resistance of plants to various external factors determines their prospects. We propose to apply the "agrochemical cocktails." It will induce the systemic plant resistance to the whole growing season, which is not possible in the case of using only one of the fungicides and bactericides. It is necessary to take into account the features of the functioning of the immune system of plants and to develop technological methods of influence on the key stages of the immune response of plants
-
Description
The article deals with the problem of oil-contaminated waste utilization. It has suggested the ways of technological advancement taking into account the principles of BAT. The analysis of the legal framework for BAT in Russia and the EU is carried out. The features of the state regulation in the field of BAT and the main approaches to formation of a complex of measures for transition to principles of BAT are considered. It has described the innovative approach to the development of waste treatment technologies that are best and available from an environmental and economic point of view. It has identified limiting parameters affecting the efficiency of waste utilization and the suitability for use as secondary material resources, particularly in the construction industry
-
INTENSIFICATION OF CULTIVATION OF CHLORELLA WITH THE USE OF IRON NANOPARTICLES
DescriptionThe influence of reduced iron nanoparticles and its oxide Fe2O3 on the growth rate of Chlorella vulgaris IFR # S-111 added to the Tamiya nutrient medium is studied. In the range of concentration of additives 0-0,1 g/l (gram per litre) an oxide inhibited the growth of microalga already when its content in the environment 6,25*10-3 g/l. With the increase of reduced iron concentration in the first twenty-four hours in the whole range the growth of the cell concentration of microalga was observed. With a maximum iron concentration of 0,1 g/l the density of a chlorella exceeded the control for 70%, and in 48 hours for 150%. The microscopy hasn't shown morphological changes of a chlorella cells with addition of the nanoparticles to nutrient medium. Accelerated reproduction of the microalga cells became the result of the intensification of the photosynthetic processes, as indicated by the nature of the parameters of delayed fluorescence (DF) of chlorophyll and shapes of the induction curves. The maximum level of DF in all experimental samples after 24 hours of cultivation was significantly higher than the control, and photosynthetic activity increased with increasing concentration of nanoparticles. In 48 hours the maximum activity was observed at concentration of nano iron 0,0125 mg/l, then decreased. It shows that with an intensive growth of a chlorella the potential of nutrient medium was quicker exhausted that led to decrease in intensity of photosynthetic processes
-
Description
The work was done in the research laboratory of the department of Biotechnology, Biochemistry and Biophysics of Kuban State Agrarian University, the aim of which was to select the most appropriate nutrient medium for maximum growth of lactic acid microorganism growth. The object of the study was its own microflora of gastrointestinal tract of quails ‒ Lactobacillus agilis, Lactobacillus intermedius and Lactobacillus salivarius. As a nutrient substrate there were used 4 of the nutrient media: the medium for lactic acid bacteria (city Uglich), the melasse-autolysis medium, the glucose-peptone medium and the MRS. During the cultivation of the microflora of the variable parameters were the time and temperature of cultivation. During the cultivation of microflora the time and the temperature of growing were the variable parameters. In the process of growing there was carried out the analysis of dynamics of consumption of reducing substances and titers of microorganisms. According to the results of growing of microbial cultures there was revealed the active consumption of carbon substrate in used variants of nutrient media, and it was found that there was observed the maximum of cells to 24 h of cultivation. On the basis of carried out results of cultivation on different media and at different parameters there was determined that the most titer of cells was reached to 24 h at the temperature 38,0 °С on the melasse-autolysis medium. So, the melasse-autolysis medium can be recommended in production conditions as the cheaper substrate at the further development of biological preparations for poultry breeding
-
INTRODUCTION OF RHODIOLA ROSEA L. IN THE CENTRAL YAKUTIA
DescriptionThe flora of Yakutia is rich in medicinal plants. Many of these plants are not still studied totally. The species Rhodiola (Crassulaceae) is one of the most popular family which are widely used by the local population for therapeutic purposes. Medicals use the rhizome and roots of Rhodiola rosea as a source of medicinal raw materials. This means the removal and destruction of plants. The procurement should be carried out solely on the basis of plants grown in culture because of the rarity and few natural reserves of the species. R. rosea L. passes a full cycle of the sprouts, blossoming and bearing fruits during the introduction in the conditions of Central Yakutia. The weather conditions of the year influence on the rate of development and growth processes very strong. The experiments determination of the germination of local reproductions were negative. The sprouts grow very good. In this case, it is good possibility of intense vegetative reproduction of Rhodiola. The evaluation of stability of introduction may be attributed to the kind of group stability. The great potential and adaptive capacity of R. rosea significantly enhances the possibility of growing this species under conditions of the Central Yakutia. It is recommended to improve and elaborate of protection of natural populations and unique plant communities
-
INTRODUCTION OF IRIDACEAE IN CENTRAL YAKUTIA
DescriptionThe results of introduction test of species and cultivars variety of irises in the Central Yakutia are described in this article. Climatic conditions of the introduction region are extreme and they are strong restrictive factor at introduction to the culture of many other species and cultivar of decorative plants. The condition of plants after rewintering was estimated on a 4-mark scale. Total number of the plants which were lost in the first winter (0 points) is 126 species and cultivars that is 60,9%. For 01.06.2015 year a collection of irises in Yakutia makes 40 exemplar of species (22 species) and 31 exemplar of cultivars (30 cultivars), 7 species of them are successfully adapted (3 points) (Iris biglumis Vahl., I. bloudowii Ledeb., I. potaninii Maxim., I. scariosa Willd., I. setosa Pallas ex Link., I. tigridia Bunge, Pardanthopsis dichotoma (Pallas) Lenz). The listed species annually plentifully blossom, form seeds and intensive form escapes. Four species Iris biglumis, I. scariosa, I. bloudowii, Pardanthopsis dichotoma are form stable self-seedling