03.00.00 Biological sciences
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CONSTRUCTION OF MUTANTS OF BACTERIOPHAGE T4 WITH REDUCED ANTIGENICITY
DescriptionA method of obtaining insertion mutants for the hoc gene, which encodes for the main phage antigen, was developed on the model of bacteriophage T4. This gene was cloned in the plasmid pBSL0+ and was disrupted by insertion of foreign DNA. The phage mutants were obtained by in vivo phage-plasmid recombination. The construction of insertion bacteriophage mutants was carried out in two stages. The resulting mutants on this procedure could be grown on wild-type E. coli strains, which is convenient for the production and use of these phages in therapy. The mutants obtained had reduced antigenicity. At the same time, the yield of the mutant strains was high when they were grown on the non-suppressor E. coli laboratory strains. A number of stages of purification of the bacteriophage mutants obtained were performed. Preparations have been studied by transmission electron microscopy and mass spectrometry. By several periodic cultivations of the mutant bacteriophages, it was shown that mutations of this type are stably maintained during more than 50 generations. T4 related bacteriophages of the family Myoviridae, for example, T-even, have the significant homology amongst their genomes, which makes possible to produce similar mutants. Thus, our method was developed to obtain mutants with reduced antigenicity which can be used for both the treatment of systemic infections, and diarrhea in the case, when, bacteriophages penetrate into the bloodstream. Such phages can be used in medicine and veterinary. The reported study was partially supported by RFBR, research projects No. 13-04-00991, 16-44-230855
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DESIGN OF THERAPEUTIC PHAGE COCKTAILS BASED ON T4-TYPE BACTERIOPHAGES: ADVANTAGES AND DISADVANTAGES
DescriptionIn the review, the stages of designing therapeutic cocktails of T4 type bacteriophages based on works by Harald Brüssow from Nestlé S.A. (Switzerland) are considered. The main stages of this process are identified: analysis of existing cocktails, selection of phages; creating a collection; cultivation of the host bacterium, multiplication of viruses; purification of the preparation; contamination testing; preservation, stabilization and storage; preclinical and clinical trials. H. Brüssow first studied the Russian drug " Coli-Proteus bacteriophage " of "Microgen" with the help of metagenomic analysis, electron microscopy and conducted its clinical studies. Prof. Brüssow considered the advantages of T4 bacteriophages for the treatment of Escherichia coli infections. Researchers studied methods of cultivation in Erlenmeyer flasks, in a bioreactor, in disposable sack cultivators for the propagation of viruses. For its purification the chromatography, centrifugation, filtration and polyethylene glycol precipitation were studied. To quickly check the contamination of phage cocktails, a mass spectrometry method is proposed. Researchers considered basic strategies, such as lyophilization, spray drying, the formation of microcrystals and microspheres to stabilize the preparations. They also reviewed the results of clinical trials of phage cocktails. We have listed the problems of selecting T4 bacteriophages from the point of view of modern knowledge. H. Brüssow and his colleagues carried out an interesting work on the construction of phage cocktails based on T4 type bacteriophages, and also revealed the problems of the current state of phage therapy
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Description
The article covers the Akabane disease agent identification in the new green monkey kidney continuous cell line (VERO) that has not been used for that in Russia before. Besides, the time for the virus detection using the fluorescent antibody method was found to be considerable decreased
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THE CONCEPT OF THE «RNA WORLD»: THEORY AND PRACTICE
DescriptionThe review examines research unusual properties of RNA. RNA has the ability to act as both genes and enzymes (ribozymes). This property could offer a way around the «chicken-and-egg» problem: genes require enzymes; enzymes require genes. Furthermore, RNA can be transcribed into DNA, in reverse of the normal process of transcription. These facts are reasons to consider that the RNA world could be the original pathway to cells. The general notion of an «RNA World» is that, in the early development of life on the Earth, genetic continuity was assured by the replication of RNA and genetically encoded proteins were not involved as catalysts. There is now strong evidence indicating that an RNA World did indeed exist before DNAand protein-based life. RNA has multiple functions. Among these, "messenger RNA" carries genetic information from DNA to protein formation. RNA is often a single-stranded spiral, but also exists in double-stranded form. In 1998, Craig Mello and Andrew Fire discovered through their studies of the roundworm C. elegans a phenomenon dubbed "RNA interference". In this phenomenon, double-stranded RNA blocks messenger RNA so that certain genetic information is not converted during protein formation. This "silences" these genes, i.e. renders them inactive. The phenomenon plays an important regulatory role within a genome. Recent years have been perhaps the most fruitful period yet in terms of research in the area of mRNA stability (Phenomena: Gene Silencing; RNA interference; Identity of mRNA decay in vivo and in vitro). The elaboration of new methods in biothechnology have been presented
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BROAD BEANS LEGUMES OF SUMMER PASTURES OF THE NAKHCHIVAN AUTONOMOUS OF REPUBLIC OF AZERBAIJAN
DescriptionThis article includes the value of forage and species composition of legume forage plants of summer pastures in Nakhchivan region. We have found that the forest, subalpine and alpine meadows and covers are regularly arranged in the vertical zone. We have also learned the species content. Flora of summer pasture forage plants rich in resources which have 1588 species (56% of the flora of Nakhchivan region)
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FORMS OF PERENNIAL GRASSES WITH RHIZOMES
DescriptionConsidering the nature of the development of sod, gemmation resumption for a period of unfavorable conditions, types of shoots, their vegetative mobility and placement in terms of ecological niches rhizomatous grasses are divided into rhizomatous, rhizome-loosely-turf and sod-rhizomatous biomorphological types, that stand out the specifics of environmental and biological characteristics forming generative, elongated, lateral shoots and roots, differing features of morphology, anatomy, biochemistry and phytocoenology forming continuous grass stand
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KRIMBAS - A NEW WINE HIGHER QUALITY MUSCAT BLACK BERRY GRAPE VARIETY
DescriptionThe variety Krimbas is a hybrid, was gotten by Panteley Zamanidi in Greece and Troshin Leonid in 2001 year by crossing of the varieties Aigeorgitiko and Muscat aleksandriisky. It is got into the group of black sea grape varieties (convar. pontica subconvar. balcanica Negr.). The production period is 146-155 days. The growth of shoots is higher. The average mass of cluster is 300 g. It has higher resistance to frost, drought and fungal diseases compare with Muscat Vitis vinifera L. varieties. The leave is middle, black-green, five lobes, deep dissected, the bubbles on the upper side of leaves surface is low. The upper and bottom cuttings are closed with gleams. The form of cut of leave with low elliptic gleam, the length of petiole is middle. The flower is hemofradit. The ovary is low conical. The pollen is fertile. The cluster is middle or round, conical, middle density. The berry is middle side, oval, black-blue color. The skin is thick. The pulp is juicy, with Muscat after taste, the juicy is not colored. The sugariness of juice is very higher: the berries after overripe faded, then overraisines till 30% from the berry. The quantity of seeds in berry one, rare two. The seed of middle length, brown color, piriform, with shot beak. There are sometimes berries with not advanced seeds. The variety used for preparing dry pink and red wines of perfect grade, so and dries higher quality desert and liker wines; it is used for preparing juice, compotes and jam. The risen got of good quality with soft Muscat after taste.
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BLOOD SUPPLY OF BIOLOGICALLY ACTIVE SPOTS OF THE THORAX OF ANIMALS
DescriptionIn the article, the review of the results of the research of blood supply of the thorax of large horned livestock, a pig and a dog is given. Blood supply and outflow of blood from biologically active spots of the thorax are discussed
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LARGEFRUITNESS OF WILD ROSE ON THE SLOPE OF THE FOOTHILLS OF KABARDINO-BALKARIA
DescriptionProducts, received from hips (fruits of wild roses), are profit a high consumer demand, that dictate solutions, which connected with technology of improvement natural dense bushes and creating artificial planting the most valuable in economic relation forms. In connection with it different characters of allocation of wild rose’s bushes is revealed by indicator of size of hips
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NUTRIENT MEDIUM FOR INDUCTION GEMMOGENESIS TO CREATE SOMATIC CLONES OF TEA PLANTS IN VITRO CULTURE
DescriptionThe article presents the first results of studies of somaclonal variability, which take place during cultivation of tea plants tissues and organs in vitro culture. As a starting material, there was used morphogenic callus, isolated from the basal part of tea microshoots, because callus increases somaclonal variability. An optimized protocol of the nutrient medium for induction gemmogenesis to product somatic clones of tea plants in vitro culture was developed. Morphogenesis calluses, initiated from the basal part of tea microshoots and subcultured on the nutrient medium with 6 – BAP – 2,5 ml + gibberellic acid – 1,0 ml + Tidiazuron – 4,0 ml + Tryptophan – 1000 mg, distinguished by high rates of induction of gemmogenesis – 63,3 %. The article studies the effect of exogenous growth regulators on morphological and growth indices of tea callus culture in vitro