№ 114(10), December, 2015
Public date: 30.12.2015
Archive of journal: Articles count 111, 292 kb
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FEATURES OF THE CULTIVATION OF PROPIONIBACTERIUM SHERMANII STRAIN
DescriptionWe have presented materials about nutrient media for growing and industrial the cultivation of Propionibacterium shermanii: Trypticase Yeast Extract Glucose Medium, the culture medium Ellinger and ATCC Medium 33. We have studied pH change and its impact on the biomass accumulation of propionic acid microorganisms. We have also defined the best time of cultivation
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Description
The article presents the results of three years of researches conducted at the Department of Viticulture of Kuban State Agrarian University, to study the processes of shoots and rooting of the three-bud cuttings of grapes of Pervenets Magaracha, Podarok Magaracha and Cytrony Magaracha of the selection of Magarach Institute of Grape and Wine. The area of these varieties, which are inter-specific hybrids, in the Krasnodar region is more than 2 thousands hectares. The cuttings were rooted in sawdust in a greenhouse on a heated rack. It was found, that the regenerative capacity of cuttings was strongly dependent on the method of their storage prior to installation on the germination. The best results were obtained in the case when cuttings had been stored in the refrigerator before starting the experiment. The lowest impact of the way to store cuttings was on the percentage of cuttings with blossoming eyes, and the largest - the length of the shoots, the output of the cuttings with at least 3 of the roots, and the average number of roots developed on one cutting. Rooting of cuttings and their growth with three and more roots were different over the years for the varieties of Pervenets Magaracha and Cytrony Magaracha, in favor of one sort or another, but the average 3-year difference between varieties was not significant. On average, in two or three years, the maximum number of roots appeared in the variety of Cytrony Magaracha, which was a bit ahead of Pervenets Magaracha. The difference in the number of cuttings formed at the roots was authentic. Correlation analysis showed that the closest relationship to the performance of the rooting ability of cuttings was found in the length of the shoots. The level and the nature of the identified correlations suggests that the rooting ability of the cuttings of the varieties of Pervenets Magaracha was determined primarily by hormonal activity, Podarok Magaracha by its supply of plastic substances, and Cytrony Magaracha had equally both factors
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FEATURES OF REGENERATIVE ABILITY IN CUTTINGS OF TECHNICAL VARIETIES OF BIANCO, VIORICA AND RITON
DescriptionThe article presents the results of three years of studies on regenerative processes in technical white one-bud cuttings of resistant varieties of grapes Bianca, Viorica and Riton. The studies were conducted in greenhouse experiments at the Department of Viticulture of Kuban State Agrarian University. It was found, that the cuttings of Viorica varieties had achieved the best ripening, and the worst was Bianca. Varieties of Viorica and Ryton which have rather good level of ripening of the cuttings, had starch in carbohydrates and Bianca variety had sugar. Earlier blooming of buds characterized the cuttings of Ryton. Blooming of the buds of Bianca somewhat delayed. Maximum length of the shoots characterized the cuttings of Bianca, and the lowest – Viorica. The shortest pre-root period (26.3 days) was observed in Ryton, and the longest (29.3 days) – in Bianca. Viorica was characterized by the highest yield of cuttings with 3 roots and more (81.3%), this indicator was a bit less (72.5%) for Ryton. For Bianca it was minimal and it was 26.3%. A similar pattern was observed for the average number of cuttings formed at the roots: the variety Viorica - 9.4 pc.,Ryton - 8.1 pc. and Bianca - 4.3 pc. Thus, cuttings of the varieties of Viorica and Ryton belong to a group with a very high root growing activity and Bianca has an average activity
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THE EFFECT OF TREATMENT OF GRAPE CUTTINGS WITH ELECTROMAGNETIC FIELD ON THEIR REGENERATIVE SKILLS
DescriptionThe article presents the results of studies on the effect of processing varieties of grape cuttings of Pervenets Magaracha using alternating electromagnetic field on their regenerative skills. Processing the cuttings with alternating electromagnetic field was carried out using an induction coil for 5,10,15 and 20 min. As a control option, the cuttings were soaked in water, as a standard option – the cuttings were soaked for 24 hours in a 0.01% solution of IAA. Rooting was performed in vessels with water at optimum temperature conditions. It was found, that the use of IAA for the first time led to the inhibition of blooming buds. EMF treatment of cuttings with each exposure has a stimulating effect on this indicator. Application of EMF exposures in the 5 to 15 minutes resulted in a significant increase in the length of shoots, compared to the control variant and the option to IAA. Processing cuttings with EMF exposures at 5,15 and 20 minutes resulted in a significant increase in rooting compared with the control. Maximum rooting was obtained at an exposure of 15 minutes. It was the same as in the version with a standard rooting stimulant - IAA. However, the highest yield of cuttings with 3 roots and ended up in a version with a 20-minute exposure. Maximum number was noted on the heel of the roots cuttings option with IAA. Treatment of cuttings with EMF exposure 5, 10 and 20 minutes, has also led to a significant increasing of this indicator, respectively, 45.5; 22.7 and 79.5%. The maximum increase in the number of roots was obtained in the variant with exposure 20 minutes. Thus, this option should be considered as the best one
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APPROBATION OF MULTIPLEX SSRANALYSIS FOR DNA-FINGERPRINTS OF RICE VARIETIES
DescriptionResults of testing of multiplex sets SSR-markers for genotyping of rice varieties are presented in the article. Two sets of SSR-markers were formed: 1: RM1+ RM11+ RM70+RM122; 2: RM164+RM167+RM168. The optimal combination of DNA markers in the multiplex sets and PCR conditions allowed obtaining accurate, easily interpretable results when performing fragment analysis on automated genetic analyzer ABIprism3130. Using multiplex sets, genotyping was performed for several varieties of rice: domestic breeding and one variety – IR36 from the breeding of IRRI (Manila, Philippines). For all the studied varieties specific SSR-fingerprints were obtained. RM 168 marker showed in domestic varieties a low level of polymorphism - one allele of 97 bp. However, at the same time, the variety IR-36, showed a second type of allele 107 bp. In addition, the loci of RM1, RM11, RM167 and RM164 have unique alleles in this variety. It is consistent with significant genetic differences of these varieties and the rest of the varieties in studied sample. The proposed SSR multiplexes are promising for use in DNA certification of rice varieties and assessment of genetic diversity
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Description
In this article, we discuss the results of the research of quality characteristics of Russian and foreign rice varieties. Possibility of using data in characterizing germplasm from collection is discussed
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INFLUENCE OF ORGANIC SOLVENTS ON WATER DISSOCIATION IN BIPOLAR MEMBRANE
DescriptionThe article discusses results of experimental research of the influence of aprotic and proton solvents on reaction rate of water molecules dissociation in the bipolar membrane MB-1 by the method of electrochemical impedance frequency spectrum. It was discovered, that addition of organic component in aqueous solutions results in significant influence on the parameters of water dissociation in a bipolar region of the membrane. The reason for this influence is the reduction of the mass fraction of water in solution and, consequently, in a bipolar region of the membrane, which itself reduces the rate of the dissociation reaction. Another reason for the influence of the organic solvent is its effect on the network of hydrogen bonds existing in water and aqueous solutions. Depending on the nature of organic solvent and its concentration, the network of hydrogen bonds may be strengthened, or destroyed, thus facilitating removal of the proton involved in the reactions between water molecules and catalytic centers in cation-exchange and anion-exchange layer of bipolar membrane, or retarding removal of proton. This leads respectively to speed up or slow down the rate of dissociation in the bipolar region of the membrane, as well as changing the constants of the dissociation reaction of water. Introduction of organic solvent in solutions, which are in the contact with bipolar membrane, is a convenient method of investigating the role of solution composition on the rate of proton transfer between water molecules and catalytic centers in the membranes
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INFLUENCE OF HEAVY METALS HYDROXIDES ON WATER DISSOCIATION IN BIPOLAR MEMBRANE
DescriptionThe results of study of bipolar membrane – analogue of MB-2, modified with chemically introduced chromium (III), iron (III) and nickel (II) hydroxides by the method of frequency spectrum of electrochemical impedance, by infrared spectroscopy and scanning electron microscopy in combination with X-ray spectrum analysis are presented. It is shown, that sequential treatment of cation-exchanger, contained in cationexchange membrane, with metal salt solution and alkali solution does not result in formation of complex compounds of these metals with ionic groups of ion exchanger. It was found that in these conditions the presence of heavy metals in the phase of cationexchanger confirmed by X-ray analysis, however, crystals of hydroxides of heavy metals are not detected in the size range of 1000 nm to 20 nm. These heavy metal compounds are thermally unstable and their catalytic activity in the reaction of dissociation of water molecules decreases with increasing temperature during heat treatment. The introduction of low-soluble hydroxides of d-metals (chromium (III), iron (III), nickel(II)) by chemical method can significantly improve the electrochemical characteristics of a bipolar membrane. The most effective catalysts in water dissociation reaction are the hydroxides of chromium (III) and iron (III) and, as a consequence, membranes with these hydroxides have a lower value of overpotential compared with original membrane at the same current density
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Description
A number of thermoreactive oligomers was obtained via the Michael interaction of molar excesses of hexamethylene-bis-maleimide with 5,5’-bisbenzotriazoles in melt. The structure of them was proved by the 13C spectroscopy method. The one-step synthesis in melt, without by-products evolution, in absence of organic solvents, additional reagents, and catalysts is the more acceptable in point of view of technology (a finished reaction product does not require any purification). The process itself is the nucleophile addition across double bond, and so it can be attributed to the atom-saving technologies. According to data of the dynamic scanning calorimetry (DSC) and the thermogravimetry (TG), oligomers fuse at 130.8–135.6 °С, cross-link at 185–250 °С, and begin their thermal destruction in air at temperatures of 400– 420 °С. The tensile strength at shear of the glued joints on base of oligomers (steel plates overlapped) is 14.2– 23.7 MPa (142–237 kgf/cm2 ). The positive effect from the introduce of 5,5’-bis-benzotriazolyl fragments into structure of bismaleimide thermoreactive oligomers on strength of the glued joints between metal plates was revealed
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01.00.00 Physical-mathematical sciences
MATHEMATICAL THEORY OF RATINGS
01.00.00 Physical-mathematical sciences
DescriptionWhen developing management solutions with the aim of joint consideration and comparison of various factors, partial removal of uncertainty is widely used ratings. In the theory of decisionmaking in almost the same sense, we use the terms "composite index" or "integrated indicator". The article is devoted to the mathematical theory of ratings as tools for studying socio-economic systems. We considered, primarily, linear ratings which is a linear function from a single (private) indicators (factors, criteria), constructed using the coefficients of importance (weightiness, importance). The study discusses the factors affecting the magnitude of the ratings. Three groups of causes affect the value of a line ranking: the ways of measurement of individual indicators, the choice of the set of indicators; the values of the coefficients of importance. We considered binary ratings when the rating takes two values. To compare the proposed rankings we use a new indicator of the quality of diagnostics and prognostic power. Significantly, in many managerial situations, significant differences between objects are identified using any rating. According to the fundamental results of stability theory, the same source data should be processed in several ways. Matching findings, obtained using multiple methods, likely reflect the properties of reality. The difference is the result of a subjective selection method. When using the results of the comparison of objects according to several indicators (criteria ratings), including in dynamics, very useful is the selection of the Pareto set. We discuss the examples of the application of the decision theory, expert evaluations and rankings when developing complex technical systems