№ 134(10), December, 2017
Public date: 29.12.2017
Archive of journal: Articles count 112, 287 kb
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CONSTRUCTION OF MUTANTS OF BACTERIOPHAGE T4 WITH REDUCED ANTIGENICITY
DescriptionA method of obtaining insertion mutants for the hoc gene, which encodes for the main phage antigen, was developed on the model of bacteriophage T4. This gene was cloned in the plasmid pBSL0+ and was disrupted by insertion of foreign DNA. The phage mutants were obtained by in vivo phage-plasmid recombination. The construction of insertion bacteriophage mutants was carried out in two stages. The resulting mutants on this procedure could be grown on wild-type E. coli strains, which is convenient for the production and use of these phages in therapy. The mutants obtained had reduced antigenicity. At the same time, the yield of the mutant strains was high when they were grown on the non-suppressor E. coli laboratory strains. A number of stages of purification of the bacteriophage mutants obtained were performed. Preparations have been studied by transmission electron microscopy and mass spectrometry. By several periodic cultivations of the mutant bacteriophages, it was shown that mutations of this type are stably maintained during more than 50 generations. T4 related bacteriophages of the family Myoviridae, for example, T-even, have the significant homology amongst their genomes, which makes possible to produce similar mutants. Thus, our method was developed to obtain mutants with reduced antigenicity which can be used for both the treatment of systemic infections, and diarrhea in the case, when, bacteriophages penetrate into the bloodstream. Such phages can be used in medicine and veterinary. The reported study was partially supported by RFBR, research projects No. 13-04-00991, 16-44-230855
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FEEDING CONCENTRATES AND PREMIXES FOR CATTLES
06.00.00 Agricultural sciences
DescriptionThe article gives an overview of the current developments of scientists of the Russian Federation in the field of production of feed concentrates and premixes for cattle. A critical analysis of feed concentrates and premixes of preventive and compensatory action for the control of mycotoxicoses was carried out, and their brief characteristics were given. The characteristic of biologically active substances and fillers, declared in the recipes of feed concentrates and premixes, is given. It is shown that the alternative to existing feed concentrates and premixes on the market is vitamin-mineral fodder concentrate "Tetra +", developed by the authors. Based on the analysis, a conclusion was made about the relevance of studies to identify the effectiveness of vitamin-mineral fodder concentrate "Tetra +" in livestock
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LANDSCAPE PLANNING ORGANIZATION OF THE CAMPUS TERRITORY OF PETROZAVODSK STATE UNIVERSITY
06.00.00 Agricultural sciences
DescriptionТhe results of the evaluation of the condition of trees and shrubs in various types of park and garden stands located on the territory of several educational buildings of Petrozavodsk state university are presented in the article. The details of the planning of sites and the spatial location of trees and shrubs are displayed. The species composition and the distribution of plants according to the categories of viability are identified. The 3D computer models of the studied territories with inventory results are created. The analysis of the plantation allows us to offer projects on formation of the landscape planning structure of the university campus of PetrSU
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10.00.00 Philological sciences
DescriptionThe article is devoted to the consideration of lexical and stylistic problems of political media discourse translation. The work compares the English-language fragments of the political discourse of the media and their translations into Russian in order to identify features of the translation of linguistic means, taking into account their pragmatic potential. The article emphasizes the manipulative potential of the political media discourse with the aim of influencing public opinion, forming the necessary attitude of the masses to power, lexical and stylistic features of the texts of the political discourse of the media are revealed to realize this potential. The theoretical significance and novelty of this study is due to the greatly increased interest in the study of the political discourse of the media in the aspect of translation, since the political discourse of the media reflects social and political events in the life of society, while an adequate translation of the political discourse of the media ensures the creation of the foreign political image of the state and its political leaders. It is concluded that the translator should not only correctly choose the lexical and -stylistic means in the target language, but also take into account non-linguistic factors when choosing strategies and tactics of translation. Among the main strategies for translating political media discourse, one can note the selection of equivalents and functional analogs, compensation and generalization of meanings
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FOREST DIVISION AND STATUS OF FOREST ECOSYSTEMS OF THE TAMAN PENINSULA
06.00.00 Agricultural sciences
DescriptionThe article presents long-term studies of afforestation on the Taman Peninsula. The directions of improving the ecology of the peninsula are considered by increasing the total biomass of the biogeocenosis of the given region by creating forest plantations, increase of forest cover and involvement of non-generating land in the economic circulation. Practical agro-forest-meliorative aspects of development of a specific category of nonproducing lands are presented. The basic agrotechnical methods are recommended at creation of forest plantations. The analysis of the state of forest plantations on the unique site of the Taman Peninsula is given - it is a tract of Sad Yakhno
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19.00.00 Psychological sciences
DescriptionIn the article, personal features of self-regulation of the subject are considered. Training activity of the students is investigated. The results of the research demonstrate contribution of the selfregulation features and with them related other personality characteristics (self-efficacy, viability) to the processes of student self-organization in context of choice of a learning task. Differences in self-organization strategies (adaptation-aimed and non-adaptive self-organization) are shown, with different personality characteristics. A connection between the self-organization strategies and their regulatory features is shown, with the choices made by the subject. Personal characteristics are identified, which represent a psychological resource of a sustainable adaptive self-organization strategy, allowing making and realizing the choice in favor of a new/complex situation
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MATERIAL STIMULATION OF WORK OF AGRICULTURE WORKERS
DescriptionIn the article, general question of material stimulation of workers are considered. There are given the definitions of concepts "interest", "motive", "motivation", "stimulus", "material stimulation", shown the main problems that are related to the motivation of workers and employers, whose solution is closely related to the successful, realization of the program for the development of the agro-industrial complex. The main reason for the shortage of labour in agriculture is a low level of wages that does not meet the needs of the population. Forms and wage systems in agriculture are justified by the features of agricultural production, payments in kind are applied. In connection with the policy of import substitution carried out by the state, the out flow labour from rural areals hoes decreased. To increase the efficiency of agricultural production it is necessary to carry act various measures including on stimulating workers of the agrarian sphere. It is expedient to divide wages into the main and additional parts while the guaranteed and variable share varies depending on the quality of labour. The use of new incentives for labour activity would make it possible to build a more effective labour incentive system with the use of which the employee will be an interested commodity producer, the owner of the products and resources
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Description
In this article, we show the necessity of formation of a new concept "the sustainable development of qualitatively new economy of the pre-business structures", which emphasize human capital, knowledge economy and scientific and technological progress with access to new technological way. The author substantiates the necessity of using a systematic approach to quantitative and qualitative evaluation of sustainability of small and medium enterprises. The article describes three types of economic goals of small and medium enterprises, the achievement of which is aimed at sustainable development. The main emphasis in the sustainable development of small and medium enterprises is done on a new quality of economic growth. We have developed a methodology for assessing the sustainability of development of small and medium business organizations. The proposed method of multicriteria evaluation of the pace and quality of economic growth of small and medium-go business to identify the priority criteria and determining the factor indicators. Summarizing the conducted researches allowed the author to establish that when combining the categories of sustainability, quality of growth and efficiency of business activities in a single integrated system it is necessary to consider the whole set of conditions and factors of the business environment to achieve set goals in the individual subsystems
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METHODOLOGICAL APPROACHES TO THE FORMATION OF THE DATABASE BASED ON THE ICONOGRAPHY OF PLANTS
DescriptionThe article considers methodological approaches to creating a database of the agricultural crops iconography. At present time, involving in the educational and scientific process the artistic works, as a resource of visual information, becomes a very actual and even indispensable tool for the formation of new knowledge about cultivated plants. The work deals with iconography as a system of images of certain subjects and characters presented in painting, ceramics, mosaic, sculpture, tapestry, murals, stamps, postcards, posters, labels, photos giving information about this or that agricultural plant. Electronic databases are the kind of information portal with visual images. Databases for the iconography of agricultural plants are successfully used in the scientific process, because exist the e evidence of changes in plants under the influence of scientific selection, the search for rare and ancient genotypes. Any source of information contains only a certain set of information, far from exhausting existing points of view, statistical data, analytical calculations relating directly or indirectly to this topic. Due to this circumstance, there is a need to search for new forms of information that can be successfully implemented in the iconography of the image of agricultural plants. The creation of electronic databases includes several stages. Development of the database structure. If this is a separate topic, then the basic questions of the topic are revealed and saturated with visual images. Selection of visual images. As visual images we can consider paintings of artists, cartoons, drawings, schemes, infographics, i.e. illustrations of various kinds
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Description
Production and processing of grains formed in the national economic system of the country a number of cereals-governmental sectors, such as grain production, grain elevator industry, flour, cereals and mixed fodder production, which constitute the grain complex country. The significance and role of the grain as a commodity in the state economy can not be overestimated. This product is totally liquid, which has a constant, steady demand at any time of the year, in any region. Ongoing measures to increase grain production and improve its implementation did not have a complex character, therefore, insignificant effect on the efficiency of the industry and the competitiveness of grain production. The shortage was covered by imports. According to the characteristics of management in agriculture, it should be emphasized that the absence of objective and timely information at all stages of production of the plant-breeding, and as a result, non-optimal choice of technology of cultivation of agricultural crops, might result in the fact that the cost of labor and material resources increases significantly, the company does not receive profits, and sometimes suffers losses. When selecting cultivation technology for agricultural crops, an agronomist has a database of more than a hundred times-personal of alternative technologies for each crop. It is up to the decision-maker (DMP) to find specific criteria to select the most suitable (for the owners and the climatic zone) technology of cultivating for the culture. These circumstances explain the relevance of in-depth research of economic and mathematical models and methods of analysis and evaluation of the economic efficiency of technologies of cultivation agricultural crops. The article deals with the process of developing a complex of mathematical models and methods for evaluating alternative technologies for agricultural production (using the example of crop production), their software implementation, and the main scientific results of the project