North-Caucasus Research Institute of Animal
Author list of organization
List of articles written by the authors of the organization
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06.00.00 Agricultural sciences
DescriptionThe aim of the research was to study the impact of starter complete feed (CF) with different levels of crude fat on the chemical composition of muscle tissue and liver of young geese. During days 5-28 the poultry was fed CF with 5.1-8.1 % crude fat, from 29- to 60- day age – with 5.4 %. By increasing the level of crude fat in starter CF there was a trend to an increase of the phosphorus content in muscle samples of geese hip and thigh - by 4.4-9.6 %, in the liver - by 27.2-53.5 % (P≤0,05). The chest muscles of the young geese fed high-calorie CF had 2.8-6.0 % higher fat content, than that of counterparts in the first group. At the same time, there was a tendency to a decrease in the concentration of fat in the muscles of the hip and thigh - by 1.9-5.0 % (P˃0,05). Increased level of crude fat in CF did not have a negative impact on the accumulation of heavy metals in the muscle and liver of geese, the concentration of the studied elements was within the permissible level established for food products
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METHOD OF INCREASING THE PRODUCTIVITY OF BROILER CHICKENS THROUGH THE USE OF FUNCTIONAL ADDITIVE
06.00.00 Agricultural sciences
DescriptionIn the experimental work, we studied the effect of the inulin prebiotic on the growth, development and efficiency of growing broiler chickens of the Isa cross. In accordance with the scheme of the experiment, the first control group was fed a complete feed (CF) by periods of growth. In the second group, inulin was added to the CF for the first 21 days of growth. In the third group prebiotic was added to the CF at the same rate throughout the whole period of rearing the chicks (42 days). In the second and third groups, we found the tendency to increase the intensity of growth rate in poultry by 1.6 and 3.0%, respectively, compared with the control. Feed costs for weight gain were decreased by 2.1-2.7%. In the chime of the blind processes of the intestine of the chickens of 2-3 experimental groups it was found the reduction of staphylococci and enterococci CFU, while similar to the control group lactobacilli CFU. The muscle tissue in the chickens of experimental groups had greater protein content. The optimal range of indicators is the use of inulin for the first 21 days of rearing poultry. Because of the production audit, an increase in live weight of chickens was found, in the experimental group by 2.7% (p ≤ 0.05), a decrease of feed costs by 7.5%, increase of the survival rate of poultry by 2.0% and profitability by 3.1%
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ISOLATION AND SELECTION OF THE NON TRANSDUCING E. COLI BACTERIOPHAGES FOR ANTI-COLIBACILLOSIS DRUGS
DescriptionThe isolation of E.coli phages from samples of natural and waste water obtained during expeditions in the different regions of Russian Federation was carried out. The obtained phages (286 isolates) were tested for their ability to lyse the pathogenic strains of E. coli – pathogenic agents of pig colibacteriosis in Krasnodar region. The study was conducted of their ability to phage transduction, the molecular-genetic characterization and biotechnological parameters of selected bacteriophages. For first experimental design of veterinary drugs was selected 5 coliphages having no ability of plasmids transduction. It has been shown that all the investigated phages are representatives of T4-type phages of family Myoviridae. The reported study was partially supported by RFBR, research projects No. 08-04-99111, 09-04-10132, 16-44- 230855
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THE APPLICATION OF THE BACTERIOPHAGES TO COMBAT COLIBACILLOSIS AND CAMPYLOBACTERIOSIS IN POULTRY
DescriptionThis work considers the needs and methods of phage therapy of two bacterial infections in poultry. The review examines the use of bacteriophages, from the moment of their discovery to the present day. We discuss the relevance of such diseases as compilobacteriosis and colibacteriosis transmitted from birds to humans, we show how to use bacteriophages and their efficacy in models of adult birds and chickens, including information provided on the pilot study of phage therapy at a poultry farm. The reported study was partially supported by RFBR, research projects No. 16-44-230855 and No. 13-04- 00991 and № 16-44-230855-р_а
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INCREASING OF BIOLOGICAL POTENTIAL IN POULTRY DUE TO THE USE OF PROBIOTICS
06.00.00 Agricultural sciences
DescriptionTo improve the quality, safety and competitiveness of the domestic raw materials of animal origin the use of probiotics in the poultry industry is a priority and promising direction of the development of organic agriculture. One of the key problems of the production of probiotics is to provide actively stable preparations. Biocompatibility of the indigenous microflora with probiotics, medical form and individual range of antibacterial activity of probiotics have a significant impact on the introduction of probiotics in new habitat conditions and its effectiveness. Consideration of these factors can enhance the effectiveness of probiotics. Currently, the available literature has insufficient justification of certain doses of administration and methods of using different probiotic preparations. The materials present and summarize the results of the research concerning the use of probiotics in the poultry industry, based on the achievements of Russian and foreign scientists. In addition, based on the analysis of various sources of scientific literature, we suggest ways to solve the problem of probiotic therapy and give some recommendations on the organization of the technological process of selection and application of different probiotics in poultryfeeding. Raw meat from poultry reared with the use of probiotic preparations is safe, because metabolites of probiotic microorganisms are not accumulate in the body tissues. Researches of biosynthesis regularities of probiotics were made at the cellular level, including genetic aspects, features of regulation of secretion, dependence on the cultivation conditions, interaction with binding sites on the cell surface, participation in the vital activity of the population of individual cells. The use of probiotics contributes to normalization of the intestinal microflora, activation of protein metabolism, productivity increase, reduction of feed costs per unit of production and increasing of poultry industry economic efficiency as a whole
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THE QUALITY OF THE MUSCULAR TISSUE OF YOUNG GEESE
06.00.00 Agricultural sciences
DescriptionThe article presents the materials of research on studying of influence of various levels of crude fat in the complete feed (CF) for the young geese of Lindovskaya breed on chemical composition and organoleptic quality of goose meat. In the experimental groups we increased the concentration of crude fat in the start and finish of the CF to 7.4 % and 6,85 %, respectively. As the lipid supplement, we used sunflower oil. It is established that muscle tissue of geese, consuming finishing CF with sunflower oil, has a more pronounced taste, odour, tenderness and juiciness. We may highlight the tendency to increase the proportion of monounsaturated oleic acid, in the background of the decrease of specific weight of palmitic acid in the fat muscle 60 day goslings who received the start and finish of CFs with the addition of sunflower oil
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DESIGN OF THERAPEUTIC PHAGE COCKTAILS BASED ON T4-TYPE BACTERIOPHAGES: ADVANTAGES AND DISADVANTAGES
DescriptionIn the review, the stages of designing therapeutic cocktails of T4 type bacteriophages based on works by Harald Brüssow from Nestlé S.A. (Switzerland) are considered. The main stages of this process are identified: analysis of existing cocktails, selection of phages; creating a collection; cultivation of the host bacterium, multiplication of viruses; purification of the preparation; contamination testing; preservation, stabilization and storage; preclinical and clinical trials. H. Brüssow first studied the Russian drug " Coli-Proteus bacteriophage " of "Microgen" with the help of metagenomic analysis, electron microscopy and conducted its clinical studies. Prof. Brüssow considered the advantages of T4 bacteriophages for the treatment of Escherichia coli infections. Researchers studied methods of cultivation in Erlenmeyer flasks, in a bioreactor, in disposable sack cultivators for the propagation of viruses. For its purification the chromatography, centrifugation, filtration and polyethylene glycol precipitation were studied. To quickly check the contamination of phage cocktails, a mass spectrometry method is proposed. Researchers considered basic strategies, such as lyophilization, spray drying, the formation of microcrystals and microspheres to stabilize the preparations. They also reviewed the results of clinical trials of phage cocktails. We have listed the problems of selecting T4 bacteriophages from the point of view of modern knowledge. H. Brüssow and his colleagues carried out an interesting work on the construction of phage cocktails based on T4 type bacteriophages, and also revealed the problems of the current state of phage therapy
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CONSTRUCTION OF MUTANTS OF BACTERIOPHAGE T4 WITH REDUCED ANTIGENICITY
DescriptionA method of obtaining insertion mutants for the hoc gene, which encodes for the main phage antigen, was developed on the model of bacteriophage T4. This gene was cloned in the plasmid pBSL0+ and was disrupted by insertion of foreign DNA. The phage mutants were obtained by in vivo phage-plasmid recombination. The construction of insertion bacteriophage mutants was carried out in two stages. The resulting mutants on this procedure could be grown on wild-type E. coli strains, which is convenient for the production and use of these phages in therapy. The mutants obtained had reduced antigenicity. At the same time, the yield of the mutant strains was high when they were grown on the non-suppressor E. coli laboratory strains. A number of stages of purification of the bacteriophage mutants obtained were performed. Preparations have been studied by transmission electron microscopy and mass spectrometry. By several periodic cultivations of the mutant bacteriophages, it was shown that mutations of this type are stably maintained during more than 50 generations. T4 related bacteriophages of the family Myoviridae, for example, T-even, have the significant homology amongst their genomes, which makes possible to produce similar mutants. Thus, our method was developed to obtain mutants with reduced antigenicity which can be used for both the treatment of systemic infections, and diarrhea in the case, when, bacteriophages penetrate into the bloodstream. Such phages can be used in medicine and veterinary. The reported study was partially supported by RFBR, research projects No. 13-04-00991, 16-44-230855
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PREVENTION OF VIOLATIONS OF METABOLISM IN FRESH COWS
06.02.00 Veterinary and Husbandry
DescriptionThe main diseases of lactating cows occur in the first two months of lactation, i.e. during the production peak of productivity. They are caused by the changes in metabolism during the transition period, which are not supported by adequate changes in the organization of feeding, or rather sufficient to ensure cows with nutrients, which causes a number of closely related diseases. To allocate any of them is not possible, as their manifestation depends on a number of reasons, and depending on situation may prevail one or the other. Among the most common diseases, there are ketosis, milk fever (paresis of maternity), dysplasia of the abomasum, acidosis, mastitis, endometritis, laminitis and leukemia. These diseases cause the greatest problems in herds with high productivity, and are caused by changes in metabolism in cows in the transition period and the inability to properly feed the animals at this time. To identify the causes of these diseases we do not need to study each of them individually - it is enough to deal with the peculiarities of metabolism. This period includes the month before calving and the first or second month of lactation, but the most important are 3 weeks before calving and 3 weeks later. In order to prevent metabolic disorders there were developed and tested feed for feeding cows before calving and immediately after calving. Their effect was studied on milk production and health status