State Scientific Organization North Caucasian Regional Research Institute of Horticulture and Viticulture of the Russian Academy of Agricultural Sciences
Author list of organization
List of articles written by the authors of the organization
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Description
The pathogenic agent of apple scab disease, Venturia inaequalis (Cooke) Winter, causes significant damage to the industrial production of apples. Studying the pathogen in pure culture is important for solving the theoretical and practical issues of its biology. The sporulation ability of the Venturia inaequalis laboratory culture is an important diagnostic feature in morphological culture analysis, in the test for sensitivity to fungicides, when creating a collection inoculum for an artificial infection. We have studied the morphological features of conidiogenesis of pure V. inaequalis culture, such as the location of conidiogenic structures and their form. For the first time in the laboratory culture of the pathogen, sporulation in the thickness of agar on the substrate mycelium was demonstrated. Conidiogenesis proceeded according to the blastic-annellidic type. Depending on the location of the conidiogenic structures on the aerial or substrate mycelium, their morphology was different. Conidiogenic areas in the substrate mycelium could be observed with the unaided eye, in the form of hyphal grit, while they were conidia conglomerations in each annelid. Annellide had a curved shape. On the aerial mycelium, annelids were straight and always had only one conidium. Differences in the morphology of conidiogenic structures are supposed to be related to the physical conditions of the environment in which sporulation takes place. In the agar, each mature conidium remaining at the apex of the annelid interferes with the formation of the next one, which results in its bending. The fixed arrangement of conidiogenic structures and forming conidia in the thickness of agar allows the use of substrate sporification for model studies of the conidiogenesis process
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Description
Results of the research on ecological safety of utilizing high clearance tractors for cultivation and harvesting of high stalk plants are presented. Purpose of the research was to decrease damaging of high stalk plants during multi-pass harvesting by tractor parts. For increasing efficiency and competitiveness of high stalk plants decreasing prime cost of production, increasing profitability and labor productivity are required. This can be achieved by introducing complex factors, which together with other factors as complex technological and technical modernization are required. Basic direction of modernization is utilizing completely new technologies and renewal of machines. Modern tractors utilized for tobacco growing have been analyzed. Basic demands for high clearance tractor which can be used for tobacco, grape and fruit trees growing have been developed. Researches on studying the tobacco plant folding during harvesting have been carried. Scheme of high clearance tractor MTZ-80 which can be assembled with machines for multi-pass harvesting of high stalk plants without their damaging has been developed. New scheme for modernization tractor MTZ-80 into high clearance model has been proposed. It can be done by changing speed reducers and forward wheels with size 11,2x20 on size 11,2x28. This will increase clearance from 470 to 840 mm
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ДНК-ПАСПОРТИЗАЦИЯ СОВРЕМЕННЫХ РОССИЙСКИХ СОРТОВ РИСА С ПРИМЕНЕНИЕМ SSR-МАРКЕРОВ
DescriptionIn the presented study, we have performed genotyping of modern Russian rice cultivars using microsatellite DNA-markers. The markers showed different level of allelic polymorphism: from 2 to 8 alleles per locus. For all studied cultivars,unique DNA-fingerprints were obtained
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Description
Professional apple gardening is bound to particular risks, of which is essential losses of a harvest because of diseases. An apple scab, the caused Venturia inaequalis (Cooke) G. Winter, brings the greatest loss. The main approach in monitoring of a scab of an apple-tree is creation of grades, steady against pathogen. In the present work we have carried out phytopathology testing of generations of the seedlings received from the free pollination of six forms of a crab of Malus orientalis from a collection MOS VIR (Maykop) characterized by a relative resistance to the apple scab in 2-3 classes of damage by long-term data. For infection we used inoculum, consisting both of natural population of a scab, and of strains of pathogen of various cultivars and geographical origin. When carrying out padding infection increase in force of an infectious background that can be bound to selection of the plants of biotypes of a fungus that are most adapted for genotypes was noted. It is recommended for precise elimination of unstable plants at selection at early stages of an ontogenesis to carry out more than one serial infection during the season. By results of the phytopathological testing, we highlighted three Malus orientalis forms from the six studied No. 17982, 17985 and 3080 the most perspective stability genes for an introgressiya in a cultural gene pool of an apple tree
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SYSTEM-WIDE INDICATORS AND ASSESSMENT OF STABILITY OF REPRODUCTION PROCESSES IN BRANCH PRODUCTION
DescriptionMethodical approaches to assessment of stability of reproduction processes which cornerstone formation of system of the balanced indicators which is based on coordination of functional and system-wide stability is are developed. The system of estimated indicators of stability is offered. The analytical model of assessment of stability providing the choice of criteria of efficiency and formation of system of estimated indicators is developed, as well as the analysis of dynamics of the external influencing factors, productive and economic indicators, financial and economic indicators; identification of the most characteristic and significant functional interrelations and dimension of interference of factors; assessment of reproduction opportunities of subjects of branch business. Calculation of an integrated indicator of financial and economic stability is performed. The complex assessment of stability providing combination of private indicators of eco-economic, technological and economic, financial and economic stability in an integrated indicator by means of methods of multidimensional correlation is given. The analysis of the factors influencing the level of stability and efficiency allowing estimating reproduction opportunities of the economic entities making grapes and wine-making production to define interrelations and interferences between resultant indicators and factorial signs and also to designate the arising disproportions is carried out. The generalizing characteristic of the macroeconomic, market and production factors influencing stability of reproduction processes in industrial wine growing is given. Based on the carried-out analysis of the factors causing stability and efficiency of reproduction processes in industrial production of grapes, the main reasons reducing stability of branch production are established
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06.00.00 Agricultural sciences
DescriptionThe article presents brief results of the introduction of Hibiscus syriacus L. varieties for a signs collection in the south of Russia. Since 2007, Federal State Budgetary Scientific Institution North Caucasian Regional Research Institute of Horticulture and Viticulture (Krasnodar) has been working on the introduction and researching of Hibiscus Syriacus varieties and signs collection is being formed. Introduced varieties of hibiscus are obtained in zones with a moderate temperature-humidity regime, therefore not all are easily adapted in the conditions of the south of Russia. According to the results of the research, several varieties of sources of valuable traits have been identified. Varieties with pure white petals: Totus Alba, William R. Smith, White Chiffon, White Pillar. Varieties with bright crimson color petals: Freedom, Carneus Plenus, Woodbridge, Duc de Brabant. The Blue Chiffon variety is the source of bright blue petals, Sanchonyo is a source of bright purple-crimson petals. Of great value are varieties that have an unusual combination in the color of petals, like the varieties Monstrosus and Hamabo. By the sign: a large flower (d of a flower more than 12 cm), the collection includes varieties White Chiffon, Chaina Chiffon, Pink Giant. Variety Dorothi Crane and hybrids: T-16-11, T-7-11, K-16-12 are identified as sources of round, wheel-shaped flower. With a double flower type, the varieties are represented by varieties: Blue Chiffon, White Chiffon, China Chiffon, Leopoldii, Speciosus, Lucci, Sanchonyo, Freedom, Carneus Plenus. Varieties: Woodbrige, Russion Violet, Ledy Stenly, White Chiffon, China Chiffon, Purple Pillar are sources of abundant long (more than 3 months) flowering. With a high degree of self-purification after flowering, varieties are distinguished: White Chiffon, Sanchonyo, Purple Pillar, White Pillar, Freedom. Varieties: China Chiffon, Freedom, Lucii, Leopoldii, RussionViolet, hybrid forms: T-16-11, T-18-11, T-21- 12 are characterized by increased adaptability to abiotic factors in south of Russia. Dedicated varieties and forms - sources of valuable economic and biological characteristics are of interest for involving in selection and allow to model varieties with given properties
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06.00.00 Agricultural sciences
DescriptionThe results of the study of physiological and biochemical parameters of plum plants, changing when infecting plants Plum pox potyvirus (PPV) are shown. It was found that the infected leaves at the stage of full development of the content chlorophyll a reduced by 30 % in variety Stanley and by 6 % in Kabardinskaya rannaya, chlorophyll b in less than 49 % at Stanley, 37% in Kabardinskaya rannaya, carotenoids are less than 22 % in the variety Stanley and by 11% in the variety Kabardinskaya rannaya . Leaves infected with the virus pox (PPV), in comparison with not infected, the amount of protein were decreasing: in the variety Stanley by 21 %, in the variety Kabardinskaya rannaya by 28 %. In the period of active growth of the leaves change in the content of pigments and protein is more intense. The content of lignin in the tissues of leaves, infected with the virus pox (PPV), in comparison with not infected in the variety Stanley was below on 13 % and in the variety Kabardinskaya rannaya - 8 %. The total content of water of infected tissue on the stage of a full-time development is reduced in patients leaves as compared with not infected 11 % in the variety Stanley and by 1 % in the variety of Kabardinskaya rannaya. Throughout the period of development of foliage studied parameters vary depending on the variety
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APPROBATION OF SSR-ANALYSIS FOR DNA-IDENTIFICATION OF COMMERCIAL WINE YEAST STRAINS
DescriptionThe study was performed to genotype some commercial wine yeast strains with SSR-markers. Five polymorphic SSR-markers were tested in a selection of 15 yeast strains. Tested SSR-markers showed a high level of informativeness as well as polymorphism and can be used further to analyze the genetic diversity of wine yeast
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Description
Microsatellite DNA markers are currently used effectively in the study of the genetic diversity of the gene pool of fruit crops and DNA certification of varieties. For plum now there is rather limited list of works on the development of this type of DNA markers. Most often, the search for new SSR-markers for this species is carried out by checking of crossreproducibility of SSR-markers developed in other species of the genus Prunus. In this study, for the 18 SSR-markers previously developed on a peach, there was performed testing and evaluation of the prospects for the use of the genotyping of plum cultivars. Testing was made on the 4 varieties of genetically distant, belonging to the 4 different subspecies of Prunus domestica L., showed the effectiveness of their use. During the study, all tested DNA-markers were grouped together in multiplex sets comprising 3-4 markers. This allows simultaneous genotyping of 3-4 loci in a single PCR reaction. These multiplex kits are available for use in the study of genetic polymorphism of species Prunus domestica L
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INTRODUCTION OF NEW GRAPES VARIETIES TO THE IN VITRO CULTURE
DescriptionClonal micro-multiplication – is this the basic method of obtaining the qualitative virus-free planting material. The high quality specificity of the explants reaction of the grapes varieties to the composition of nutrient medium requires the individual selection of the medium components for the most successful multiplication in vitro. In the article we present the results of studies on the cultivation of the grapes apexes in vitro the modified medium with the reduced content of macrocells. As a result of conducted investigations we have established that for the grapes varieties of Academic Trubilin, Artemis, Gurman Kraynova, Maria Kallas, Nizina, Petit Verdo and Traminer Black the most effective introduction into the in vitro culture (acclimatization of apexes 80- 100%) occurs on modified nutrient medium to Murasige and Skoog (1962), by being differed in terms of the content macro-elements (mG/l): NH4NO3 – 1237; KNO3 – 1425; MgSO4 · 7N2O – 277,5; KN2PO4 · H2O – 277,5, of the vitamin Of v1 – 10,0 mG/l, nicotinic acid – 4 mG/l. Remaining varieties of Kishmish luchistiy, Preobrajenie, Roshfor K and Yubiley Novocherkasska in the same medium were developed slowly and in a different way