03.00.00 Biological sciences
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ANIMAL HEALTH - CRITICAL FACTORS OF THE LIVESTOCK
DescriptionThe article provides an overview of materials of conferences on problems and prospects of the industry of cattle breeding
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SANITARY CONDITION OF TREES IN URBAN PLANTINGS (ON THE EXAMPLE OF VORONEZH)
DescriptionThe article presents a comparative analysis of the sanitary condition of green plantings in park area and street network of trees of local forest origin and exotic species
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NUTRITION AND HEALTH OF HIGH-PRODUCTIVE COWS
DescriptionIt this article, the situation in dairy practice with nutrition factors caused metabolic diseases such as ketosis, acidosis, lameness and a decline in fertility is shown. The article has the proposes on the nutrition of highly productive cows in transition period
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Description
The results of genetic variation and differentiation study of adjacent populations growing on swamp and on dry land was done using examine of ISSR DNA regions. The same diversity level and comparatively high division were shown in this article
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CONSTRUCTION OF MUTANTS OF BACTERIOPHAGE T4 WITH REDUCED ANTIGENICITY
DescriptionA method of obtaining insertion mutants for the hoc gene, which encodes for the main phage antigen, was developed on the model of bacteriophage T4. This gene was cloned in the plasmid pBSL0+ and was disrupted by insertion of foreign DNA. The phage mutants were obtained by in vivo phage-plasmid recombination. The construction of insertion bacteriophage mutants was carried out in two stages. The resulting mutants on this procedure could be grown on wild-type E. coli strains, which is convenient for the production and use of these phages in therapy. The mutants obtained had reduced antigenicity. At the same time, the yield of the mutant strains was high when they were grown on the non-suppressor E. coli laboratory strains. A number of stages of purification of the bacteriophage mutants obtained were performed. Preparations have been studied by transmission electron microscopy and mass spectrometry. By several periodic cultivations of the mutant bacteriophages, it was shown that mutations of this type are stably maintained during more than 50 generations. T4 related bacteriophages of the family Myoviridae, for example, T-even, have the significant homology amongst their genomes, which makes possible to produce similar mutants. Thus, our method was developed to obtain mutants with reduced antigenicity which can be used for both the treatment of systemic infections, and diarrhea in the case, when, bacteriophages penetrate into the bloodstream. Such phages can be used in medicine and veterinary. The reported study was partially supported by RFBR, research projects No. 13-04-00991, 16-44-230855
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Description
In the article we have conducted the estimation of breeding population of salix acutifolia. We have studied the following indicators of the measured trees: the percentage of salicylates, bark content in relation to the wood. For these two indices we have calculated the "complex" productivity of the trees. Based on that, the trees are assigned to these breeding categories: Category 1: trees with high productivity - ( 49.7 - 39.9 kg / t of raw materials) Category 2 : trees with average productivity - ( 39.1 - 33.8 kg / m raw materials) category 3 : trees with low productivity - ( 31.8 - 24.3 kg / t of raw materials)
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COMPARATIVE ASSESSMENT OF FUNGICIDES OF NEW GENERATIONS TOXIC TO DAPHNIA
DescriptionWe have assessed the effect of the new generations of fungicides on the vital activity of daphnia. The data of the chronic experiment allowed us to determine non-effective and threshold concentrations of the substances studied. The toxic effect of the xenobiotics is shown to be of different intensity. When entering water they may be dangerous, first of all, for the zooplankton that is the food base of young fish, and may have unfavorable effects on fish development
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Description
The article cites the results of our studies of species composition of moths and their trophic communication as well as alternative types of plants used by owlet moths on the Nordovy island. Also, the article presents the species composition of fauna found on the island
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CYCLICAL EFFECTS OF ACTINOMYCIN D ON THE BARLEY COLEOPTILES GROWTH
DescriptionIn cell biology, actinomycin D is shown to have the ability to inhibit transcription. Actinomycin D does this by binding DNA at the transcription initiation complex and preventing elongation of RNA chain by RNA polymerase. When soaking the seeds in a solution of actinomycin D, antibiotic blocks RNA synthesis and seed germination occurs at the expense of long-living RNA, available in the mature seed. In the article we present experimental data indicating that as storage seeds of winter barley are changing the action actinomycin D on the growth of coleoptiles. It is shown that actinomycin D at a concentration of 40 µg/ml in October reduced the growth, in December it was not authentically, and in February it was paradoxical amplified growing coleoptiles. We suggest the part of the actinomycin D fastened with growth inhibitor coleoptiles, whose structure changes during seed storage and increase the affinity of actinomicyn D to the inhibitors. At a concentration of 60 µg/ml actinomycin D effectively reduced of the coleoptiles growth. Suppression of seedlings roots growth was proportional to the concentration of the actinomycin D and exposure time
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Description
The number of microarthropods in the black soil de-creased with an increase in the number of oil. The same pattern was observed until a certain concentra-tion of lead in soil (von 250 mg / kg), after which high doses (500 and 1000 mg / kg) was recorded an in-crease in the number of microarthropods. Gamasid mites and ticks of akaroid-trombidiform complex have proven more resistant to lead, and armored mites and springtails - to oil, vise versa