03.00.00 Biological sciences
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CONSTRUCTION OF MUTANTS OF BACTERIOPHAGE T4 WITH REDUCED ANTIGENICITY
DescriptionA method of obtaining insertion mutants for the hoc gene, which encodes for the main phage antigen, was developed on the model of bacteriophage T4. This gene was cloned in the plasmid pBSL0+ and was disrupted by insertion of foreign DNA. The phage mutants were obtained by in vivo phage-plasmid recombination. The construction of insertion bacteriophage mutants was carried out in two stages. The resulting mutants on this procedure could be grown on wild-type E. coli strains, which is convenient for the production and use of these phages in therapy. The mutants obtained had reduced antigenicity. At the same time, the yield of the mutant strains was high when they were grown on the non-suppressor E. coli laboratory strains. A number of stages of purification of the bacteriophage mutants obtained were performed. Preparations have been studied by transmission electron microscopy and mass spectrometry. By several periodic cultivations of the mutant bacteriophages, it was shown that mutations of this type are stably maintained during more than 50 generations. T4 related bacteriophages of the family Myoviridae, for example, T-even, have the significant homology amongst their genomes, which makes possible to produce similar mutants. Thus, our method was developed to obtain mutants with reduced antigenicity which can be used for both the treatment of systemic infections, and diarrhea in the case, when, bacteriophages penetrate into the bloodstream. Such phages can be used in medicine and veterinary. The reported study was partially supported by RFBR, research projects No. 13-04-00991, 16-44-230855
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Description
In this article we have shown the results of studying of biologically active connections in bentonite clays, carotene containing raw materials for the purpose of receiving functional feed additive for agricultural birds
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FEATURES OF DEVELOPMENT OF NATURAL SISTEMS AND AGRICULTURAL LANDSCAPES OF THE WESTERN AZOV
DescriptionWe know, that in the development of the natural ecosystems of the Western Priazovye its biomass predominates. This ensures the maximum protection from noticeable changes of habitat. Basic succession defining a significant shift of energy flow in the direction to increase of their expenditures to maintain forming populations determined by broad specialization of natural systems on ecological niches, and their individuals have rather small size, the life cycles of many of them are very simple and short. The coastal strip of the Azov Sea has been seriously degraded due to powerful recreational load, especially in the summer. Presumably one should expect an increased anthropogenic load on ecosystems of this area. Quite obvious is a regularity of overload agroecosystem which occupies the territory between the river and other water formations. The relatively low productivity of agricultural systems of the Eastern Azov is limited by number of factors, among which the main place is occupied with the moisture, nutrients and high weediness of sown areas
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EVALUATION OF PARK PLANTING IN THE SYSTEM OF GARDENING OF PETROZAVODSK
DescriptionThe article presents the results of the state evaluation of trees and shrubs in landscaping plantings of different types situated in four parks in Petrozavodsk. The species and number of plants in good, satisfactory, and unsatisfactory states were determined. The conducted analysis of species distribution and the number of trees and shrubs in each category of state allows working out the activities for planting reconstruction
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Description
The number of microarthropods in the black soil de-creased with an increase in the number of oil. The same pattern was observed until a certain concentra-tion of lead in soil (von 250 mg / kg), after which high doses (500 and 1000 mg / kg) was recorded an in-crease in the number of microarthropods. Gamasid mites and ticks of akaroid-trombidiform complex have proven more resistant to lead, and armored mites and springtails - to oil, vise versa
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LAGENARIA - ICONOGRAPHY, DISTRIBUTION AND MULTI-PURPOSE USE
DescriptionThe article considers the multiple use of lagenaria or bottle gourd in different countries of the world. It presents the information of the different types of lagenaria that in each country have their own purposes. Attention is paid to the origin of culture, in this context, considered a variety of the use of culture. A distinctive feature of the culture is that lagenaria is one of the ancient plants that were cultivated by mankind as functional and decorative purposes. We have analyzed lagenaria using on the territory of our country. Total analysis with iconic images of plants allowed us to evaluate new opportunities in the use of culture. In the work we have applied methods of analysis, synthesis and descriptive method
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CYCLICAL EFFECTS OF ACTINOMYCIN D ON THE BARLEY COLEOPTILES GROWTH
DescriptionIn cell biology, actinomycin D is shown to have the ability to inhibit transcription. Actinomycin D does this by binding DNA at the transcription initiation complex and preventing elongation of RNA chain by RNA polymerase. When soaking the seeds in a solution of actinomycin D, antibiotic blocks RNA synthesis and seed germination occurs at the expense of long-living RNA, available in the mature seed. In the article we present experimental data indicating that as storage seeds of winter barley are changing the action actinomycin D on the growth of coleoptiles. It is shown that actinomycin D at a concentration of 40 µg/ml in October reduced the growth, in December it was not authentically, and in February it was paradoxical amplified growing coleoptiles. We suggest the part of the actinomycin D fastened with growth inhibitor coleoptiles, whose structure changes during seed storage and increase the affinity of actinomicyn D to the inhibitors. At a concentration of 60 µg/ml actinomycin D effectively reduced of the coleoptiles growth. Suppression of seedlings roots growth was proportional to the concentration of the actinomycin D and exposure time
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Description
The article cites the results of our studies of species composition of moths and their trophic communication as well as alternative types of plants used by owlet moths on the Nordovy island. Also, the article presents the species composition of fauna found on the island
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COMPARATIVE ASSESSMENT OF FUNGICIDES OF NEW GENERATIONS TOXIC TO DAPHNIA
DescriptionWe have assessed the effect of the new generations of fungicides on the vital activity of daphnia. The data of the chronic experiment allowed us to determine non-effective and threshold concentrations of the substances studied. The toxic effect of the xenobiotics is shown to be of different intensity. When entering water they may be dangerous, first of all, for the zooplankton that is the food base of young fish, and may have unfavorable effects on fish development
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Description
In the article we have conducted the estimation of breeding population of salix acutifolia. We have studied the following indicators of the measured trees: the percentage of salicylates, bark content in relation to the wood. For these two indices we have calculated the "complex" productivity of the trees. Based on that, the trees are assigned to these breeding categories: Category 1: trees with high productivity - ( 49.7 - 39.9 kg / t of raw materials) Category 2 : trees with average productivity - ( 39.1 - 33.8 kg / m raw materials) category 3 : trees with low productivity - ( 31.8 - 24.3 kg / t of raw materials)