03.00.00 Biological sciences
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ИНИЦИАЦИЯ ПРОЭМБРИОГЕННЫХ КЛЕТОЧНЫХ СУСПЕНЗИЙ У ДЕВЯТИ МЕЖВИДОВЫХ ГИБРИДОВ ВИНОГРАДA
DescriptionЧерешки листьев, взятых из растений ин витро, девяти межвидовых гибридов винограда: подвоев Берландиери х Рипариа ‘Кобер 5ББ’, Рипариа х Рупестрис ‘101-14’ и сортов (cvs): 'Бианка', 'Зигфридребе', 'Подарок Магарача’, ‘Первенец Магарача’, ‘Цитронный Магарача’, ‘Интервитис Магарача’ и гибридной формы ‘Магарач 100-74-1-5' культивировали на твердой среде NN, содержащей различные концентрации 2,4-D и BА в различных сочетаниях. Для того, чтобы инициировать рост клеточных суспензий, проэмбриогенные каллусы пересаживали в жидкую среду NN, дополненную 1.0 мг/л 2,4-Д и 0,2 мг/л BА. Субкультивирование этих суспензий в жидкую среду NN, дополненную 2 мг/л NAA и 0,1 мг/л BА, привело к развитию агрегатов эмбриоидов, а пересадка в жидкую среду с 0,5 мг/л BА вызвала развитие единичных глобулярных и сердцевидных эмбриоидов. Проэмбриогенные клеточные суспензии, состоящие из преимущественно отдельных клеток, могут быть использованы в трансформации генов и селекции на клеточном уровне с целью уменьшения вероятности появления химерных растений
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Description
Artificial reduction of photoperiod among di-and-tetroploid stevia varieties (Stevia rebaudiana Bertoni) to 12 hours in the second part of the II stage organogenesis fastens the transfer to III stage organogenesis and induces blossoming for 30-32 days earlier than at natural photoperiod. This provides stable seed productivity under field tests. Blossoming features providing crossbreeding are discussed
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Description
The article gives the description and the algorithm of the simulation model SMDS "Potok" 1.0, designed to calculate the parameters of distribution and sedimentation of technological alluviums in watercourses. The relevance of the simulation model is due to the need to determine the integral indicators (the volume of water flowing through the areas of the plume with a predetermined suspension concentration, the average layer of the silt, the specific density of the bottom silt in the calculated zone) when calculating the harm to water biological resources from the implementation of various economic activities in accordance with the methodology of calculation of damage caused to water biological resources approved by the order of the Federal Agency for Fisheries No. 1166 of 25.11.2011. Usage of the simulation model of SMDS "Potok" 1.0 will increase the reliability of calculations in determining the damage to water biological resources, as well as bring the calculations of parameters of distribution and sedimentation of technological alluviums in watercourses in accordance with the current regulatory legal acts of the Federal Agency for Fisheries
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HORSERADISH PEROXIDASE IMMOBILIZATION IN CALCIUM ALGINATE HYDROGEL AND MICROPARTICLES
DescriptionIn the article two differently obtained preparations of immobilized horseradish peroxidase are compared with free enzyme and with each other by their stability. It is shown that both preparations of immobilized peroxidase possess increased stability during storage and heating. It is established that activity of hydrogel obtained increases after heating
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Description
The study of agronomy plants history, its distribution and use through image analysis and iconography have been presented. As an object of study, the image of wheat has been taken as the ancient culture of the world among agricultural plants. After many years, the illustrative series takes on a completely different meaning, namely, it becomes an independent resource of information. The analysis includes images of wheat in painting, sculpture, mosaic, tapestries, murals, botanical illustrations, stamps, porcelain and scientific drawings. In the work, we have made an attempt to analyze the images of wheat in the works of art in order to search for species diversity, directions of selection and genetic research, and also to present the morphological characteristics of culture. The method of analysis is a method of visual notes or sketches, which consists in comparing and searching information by image. The analysis is carried out on grounds: tall wheat, multiflorous forms of wheat, nonspreading, thickness of straw. The visual analysis of the images of wheat in works of art by the method of sketches made it possible to reveal the species diversity of culture along the ear, to see the ancient forms that were cultivated in the world before the "green revolution". The analysis included works of art from the 14th to the 21st century, the masters of painting in Italy, Germany, Russia, the Netherlands, France, the United States, and others
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THE EFFICIENCY OF THE "MYCOSIM SP +" ENZYME FEED ADDITIVE IN THE DIET OF QUAIL
DescriptionThe article presents the materials on the effect of "My-cosim SP +" enzyme feed additive on growth and de-velopment, biochemical and morphological parame-ters of the quail and the results of post-mortem autopsy of quail to determine the effect of additives on changes of the quality and flavors of the meat
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Description
This article is devoted to the study of small mammal communities (species composition, daily activity and spatial distribution) in the agrocoenosis. The fields of winter crops that were surrounded by similar fields or by winter crops with meadow vegetation were researched. The Mus musculus became the dominant species in the winter crops agrocoenosis. The R. Sylvaemus living near the border with field-protective forest belts (and 25 meters field around) are subdominant group. Marked single finds Mus musculus in the forest and R. Sylvaemus in the field (at a distance before 50 meters). It is shown that the presence of meadow vegetation increases the species diversity of mice in the agrocoenosis. In the middle of this field a subdominantion group is Microtus arvalis s.l. and Cricetulus migratorius. The both species prefer a neighborhood with meadow vegetation. The method of recapture, followed by labeling with animals, made it possible to fix the movement for a distance of about 400 m. during the night (maximum values were males Mus musculus). The activity of small mammals in the winter crops was timed to coincide with the day-night cycles, with high activity from 01:00 to 04:00 am, and minimum activity after 09:00 hours of the morning before 08:00 pm
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STUDY OF SOIL ALGAE AS BIOINDICATORS IN AREAS OF PETROLEUM PRODUCTION
DescriptionAlgae as bioindicators on soil pollution by xenobiotics were studied in Krasnodar region. Explicit alteration of algacenosis was observed on the territory functioning petroleum production site, it testifies the consistent soil pollution. Alga flora of preserve area zone differentiated with high species diversity.
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Description
The article presents the materials of the study of the features of cultivation of Lactobacillus sp. on different culture medium. We have made a comparison of standard culture medium for cultivation Lactobacillus sp. and determined the most optimal culture medium
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Description
The article presents the materials of the study and selection of modes of Azotobacterchroococcum cultivation due to increasing the colony formed units (CFU) and improving the synthesis of microbial polysaccharide that prolongs the shelf life of the finished product