03.00.00 Biological sciences
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DEVELOPING OF THE METHODOLOGY FOR THE IDENTIFICATION OF PICEA ABIES L. CLONES BY USING ISSR MARKERS
DescriptionThe technology for the identification of Picea abies L. clones by using ISSR markers was developed. 9 ISSR primers ((GA)9T; (AC)8C; (CA)6RY; (CA)6RG; (CA)6(GT); (CA)6(AC); (AG)8T; (GA)8С и (AG)8YT) were recommended to use for genotypes detection. The selected primers have shown the high level of polymorphism. A total of 172 amplified fragments were found by the selected primers and 149 (86.6%) of them were polymorphic
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Description
The article considers the new method for recovery of P. arbelaeziion intact material on the basis of grafting into endogenous pathogens resistant Passiflora species in vivo. The influence of the explants origin depending on the stock of intact plants on the development of regenerated P. arbelaezii under in vitro condition is examined
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DEVELOPMENT OF OPTIMAL WAY OF OBTAINING A HYDROLYZATE OF LACTIC ACID BACTERIA
DescriptionThe work was carried out in the scientific-research laboratory of the chair of Biotechnology, Biochemistry and Biophysics of Kuban SAU, the aim of which is the search of optimal conditions of obtaining the hydrolyzates of lactic acid bacteria by thermoacid and enzymatic ways. The pure cultures of microflora of gastrointestinal tract of quails − Lactobacillus agilis, Lactobacillus intermedius and Lactobacillus salivarius were the object of researches. Under conducting the thermal acid hydrolysis there were used the same correlations of bacterial mass and water as well as procedures of clearing of hydrolyzates. The variable conditions were рН, temperature and time of hydrolysis. When carrying out the enzymatic hydrolysis in this study there was used the enzyme from the group of muramidase lysozyme. The amount of enzymes and time of carried out hydrolysis were the variable conditions under obtaining the enzymatic hydrolyzates. In the process of researches there were studied: qualitative content of total protein, peptides with М.м. < 1500, protein and peptides with M.m. > 1500, aminoacids and as well as GMDP (glucosaminilmuramilpentapeptide). On the basis of carried out experiments there was stated that according to all components of biologically active substances the thermal acid way is more effective than enzymatic. The maximum accumulation of BAA in hydrolyzates by different ways was revealed at destruction of cells Lb. intermedius. Content of GMDP in thermal acid hydrolyzate was higher than in enzymatic on 0,028 g/100 ml (51,8 %)
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Description
In the article, we have proposed regional environmental standards for heavy metals, oil and oil products in the main soils of southern Russia
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DEVELOPMENT OF A FUNCTIONAL FEED ADDITIVE
DescriptionThe new feed additives for animals based on milk whey, enriched with sprouting wheat, barley, maize grains and lactic-acid bacterium have been presented in this article. This study explores the possibility of combining the prebiotics ability of milk whey and sprouting grains with feed probiotic attributes of microbe to enhance gut health of animals and digestibility of the feed. Twelve variants of products were tested in the study using the microbial and physical-chemical approaches. All the assays showed high count of microorganism and high content of reducing sugar. The results of investigation indicate that selected feed additive show high quality. An additional point is that it contains useful organic acids (lactic, acetic and propionic acids) and 2,1×109 colony-forming unit of probiotics microorganism that hold the concentration for 4 months. There are not yeast, must, coliform bacteria and Staphylococcus aureus in the developed functional feed product. It contributes to the normalization of the microflora of the gastrointestinal tract of animal, suppression of conditionally pathogenic and putrefactive microflora. The elaborated feed component will help to provide combined feed companies with accessible high-quality raw material
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DISTRIBUTION AND VARIABILITY OF MORPHOLOGICAL TRAITS OF CENTAUREA DAGHESTANICA (LIPSKY) CZER
DescriptionThe distribution and variability of features of the endemic of flora Eastern Caucasus Centaurea daghestanica (Lipsky) Czer. were given in this article. Eleven locations of the species are detected and three of them are new ones. C. daghestanica grows in the lower and middle mountain belts from 400 to 1250 m above sea level. In the lower belt, the species is found on clayey areas of the solonchak valley of Kar-Kar and the limestone slopes adjoining the valley, on average on shale screes and stony slopes. Three populations of the C. daghestanica we studied. Species composition on the investigated sites was determined, and a geobotanical description is carried out. Project coverage of C. daghestanica in the studied populations varies within 2–5%. By generative individuals of different ages were represented mainly populations. One generative shoot from 30 individuals in each population was taken to reveal the variability of the generative shoot structure, on which 13 traits were taken into account. The degree of variability of the morphological features of C. daghestanica shoot is different, the influence of the altitude level on them is not the same. The annual shoot of C. daghestanica has an average of 3-5 branches of the first order. Branches of all levels are located at the bottom of the shoot
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PLANTS AS AN ECOLOGICAL NICHE FOR HUMAN PATHOGENIC BACTERIA
DescriptionThe facts related to contamination of plants with pathogenic bacteria being a possible source of human foodborne intestinal diseases are discussed. Data bound up with influence of environment conditions with respect to attachment and penetration of bacteria in plant tissues are described. Exquisite results related to the rate of proliferation and the time of retaining enteropathogens in plant organisms are presented
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REACTION OF APPLE-TREE VARIETIES AND STOCKS ON CHANGE OF pH OF SOIL
DescriptionThe researches are devoted to the determination of physiological parameters of grafted apple-trees joining with the level of their resistance to the change of soil рН. The experiments were carried out in the conditions of lysimetric experience with the soils use characterizing by different value of рН. The scheme of experience included the following variants of soil medium reaction: neutral (рН 7,3), typical for black soils of plain part of the region (control); weak-acid (рН 6,2) existing in soils of foothill zone of the region (grey and brown forest); strong-acid (5.1) typical for brown forest soils; weak-alkaline(рН 8.3) occurring in black soils of south and sod-carbonate soils, strong-alkaline ( рН 8.8) typical for maternal types of many black soils. There were studied the varieties of apple-trees Prima, Florina and Renet Simirenko grafted on stocks M9 and MM106 and as well as corresponding the non-grafted stocks. The reaction of grafted apple-trees on change of рН of soil is specific and depends on adaptive possibilities of grafting components: varieties and stocks. At the stock M9 we can see the active growth of shoots and roots in the meaning of pH values from 5,1 (strong-acid reaction) to 7,3 (neutral). Plants MM106 prefer neutral (pH 7,3) and weak-alkaline (pH 8,3) soils. Independently from stocks for the variety Florina the most favorable weak-acid soils and the variety Prima – mainly neutral. The variety Renet Simirenko possesses enough high and stable functional activity in the conditions of wide range of the medium reaction: from very acid to alkaline one
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Description
Researches were carried out on three-eyed cuttings of stock of phylloxera-resistant grape varieties Berlandieri×RipariaCober 5BB (Cober 5BB), Riparia× Rupestris101-14 (101-14), Berlandieri × RipariaСО-4 (СО-4), Gravesak and RSB. Cuttings of presented varieties were wetted during 24 hours in water and after preliminary drying of the surface were covered in the upper part with antitranspirant, under the temperature about 90°С. Then 40 cuttings of every variety were placed with lower ends for 24 hours in 0,01% heteroauxin solution or for 8 hours in 1% Radix Plus solution. Cuttings of the control variant were placed in water. After treatment cuttings, were couching in a film greenhouse on a heated rack in humid steamed sawdust. In the result of the present researches there was determined that the wetting of basal ends of cuttings of stock phylloxera-resistant varieties in 1% Radix Plus water solution plus during 8 hours leads to the essential activity of regeneration processes in them. The expansion of the length of shoots on rooted cuttings of the experimental variant amounted for 19,2–154,5 %, the rooting of cuttings - 23,3–76,7 %, the output of cuttings having at least three roots – 33,0–78,1 %, the number of calcaneal roots– 80,8–257,1 %. The maximum increase of the length of shoots under the influence of Radix Plus was observed at varieties of Gravesak and СО-4, the rooting – Cober 5BB and CO-4, the amount of cuttings at least three roots and average amount of roots on them –101-14 and Cober 5BB. The Radix Plus caused the great stimulating influence the rootforming ability of cuttings of stock varieties than heteroauxin, the standard stimulator of root-forming
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Description
Researches on non-root additional fertilizing of grapes with complex water-soluble fertilizer called Nutrivant plus have been conducted in corporation "Pobeda" in Temrjuksky area of Krasnodar territory on the implanted plantings of technical grade Rhine Riesling. In the test, the following kinds of Nutrivant plus were used: oleiferous (N0P20K33), sugar beet (N0P36K24), grapes (N0P40K25), universal (N19P19K19), grain (N6P23K35). Each mark of Nutrivant plus contained a certain set of microelements. The test consisted of six variants:1 variant - without additional fertilizing(control); 2 variant: grapes - before flowering, grapes - a growth phase of berries (a berry about a pea), oleiferous - the beginning of maturing of berries; 3 variant: universal - before flowering, universal - a growth phase of berries (a berry about a pea), oleiferous- the beginning of maturing of berries; 4 variant: grain - before flowering, grain - a growth phase of berries (a berry about a pea), oleiferous- the beginning of maturing of berries; 5 variant: Sugar beet - before flowering, a sugar beet - a growth phase of berries (a berry about a pea), a sugar beet - the beginning of maturing of berries; 6 variant: universal - before flowering, grain - a growth phase of berries (a berry about a pea), grapes - the beginning of maturing of berries. Norm of the expense of fertilizer is 3 kg/hectares. The expense of a working liquid - 700 l/hectares. The result was, that in the third, fourth and fifth variant of the test the increase in a crop from a bush happened accordingly on 7,5, 13,8 and 17,8 %. The maximum increase of a crop in the fifth variant was accompanied by increase in mass concentration of sugars in juice of berries on 0,6 g/100 см3и decrease titratable acidities for 0,6/dm3 As a result triple not root additional fertilizing of grapes plants grade Riesling with Nutrivant plus (universal - before flowering, grain - in a growth phase of berries and grape - in the beginning of maturing of berries) promoted more optimization of a water mode of leaves, increase in weight of a cluster, a crop from a bush and productivity, mass concentration of sugars and phenolic substances in juice of berries