03.00.00 Biological sciences
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EXTRACTING DNA BY NUCLEOSPINPLANT 2 COREKIT. COMMENTS
DescriptionThis review article highlights a new method of DNA extraction from herbarium leaves of grapes for use in PCR-analysis
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Description
In the article we have investigated the influence of nickel oxide in the amount of 100, 1000 mg/kg of the soil (1, 10 MPC), combined with the influence of an alternating magnetic field of induction of 50, 100 and 650 µT power frequency of 50 Hz on the biological properties of alkalinity black soil
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DESCRIPTION OF CERTAIN ASPECTS OF THE PRODUCTION POTENTIAL OF SOILS TAMAN PENINSULA
DescriptionThe work presents the results of the scientific research in the Taman Peninsula soil properties. We carry out the historic review of the studies in the Taman Peninsula soils, and its geological structure. We present morphological characteristics of the soil profile for the southern, calcareous, ultra heavy loam chernozem developed from the loessial loams and forming genetic horizons. The studies of the humus content in the soils show the associations between its percentage and the type and intensity of the agricultural use. Studies of the soils structure and composition demonstrate marked aggravation of the soils physical properties caused by grape growing. The best results of the particle size analysis have been achieved for the woodland soils. In woodlands, after their establishment, the soils under the grass and crown layers develop free of the anthropogenic impact. We register self-supporting growth of organic matter and natural processes of soil fertility recovery; therefore, such soils are characterized with a greater content of organic matter as compared to the soils under grape plantings cultivated in the monoculture regime. Reduced soil organic matter (humus) content in vineyards results from the disturbed biological interchange of the matter and increased anthropogenic impact causing erosion processes
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EFFECTS OF INCREASED WATER TEMPERATURES ON REPRODUCTIVE CHARACTERISTICS OF RAINBOW TROUT FEMALES
DescriptionThe effects of increased water temperatures on the reproductive characteristics of rainbow trout females were studied. It was found, that the increased water temperatures produced negative effects on the gonad maturation and survival of the embryos when acted at the late stages (IV and V) of female gametogenesis, but not at the earlier (III) stage
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Description
The article presents the results of the studies on the effect of processing grape cuttings of different lengths with Norwegian rooting stimulator called Radix plus on their regenerative capacity. In the experiment we used two-, three-, four- and five buds cuttings of Vostorg table grape variety, characterized by weak rooting activity. Paraffined and pre-processed by growth regulator, these cuttings were rooted in vessels with water. The studies found that with the increasing length of cuttings we decrease the degree of blooming buds, which is associated with the manifestation of the longitudinal polarity. Processing cuttings with Radix Plus leads to inhibition of blooming buds and shoot growth, which is enhanced by increasing the length of cuttings from two to five buds. The maximum of rooting ability of the cuttings and output with 3 or more was reached by roots of two-buds cuttings. The regularity of these parameters decrease with increasing length of the four buds cuttings with some increase in the five buds; the maximum number of roots was reached by two buds and five buds cuttings. Using of Radix Plus reduces the difference between the rooting on short and long cuttings. The most consistent effect of Radix Plus is the output of the cuttings with 3 roots and more on three buds cuttings, and on the increasing number of roots - in the three- and four buds cuttings. When germinating the cuttings of Vostorg variety in comfortable conditions, without using growth regulators, it is better to use two buds cuttings, and in the case of pre-processing them with Radix Plus – we suggest three buds cuttings
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Description
In this article, we have presented a number of modeling experiments to investigate the chemical pollution of brown calcareous soil. It assesses the stability of brown calcareous soil of a nature reserve called Utrish to contamination with heavy metals in biological parameters. Pollution of brown calcareous soil with Cr, Cu, Ni, Pb and oil causes a significant reduction in biological parameters. The degree of reduction depends on the nature of the contaminant and its concentration in the soil. The study showed that the indicators used in the biological condition of the soil, could be recommended for use for diagnosis, monitoring, and regulation of chemical pollution of brown soil. According to the degree of resistance to chemical contamination, the brown soils of Russia form the following series: typical brown - brown carbonate - brown leached
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Description
Qualitative and quantitative composition of the low-molecular carboxylic acids (CA) which were contained in the cultural liquid (CL) of the four strains of Escherichia coli and Salmonella enteritidis var.Issatchenko was investigated. The differences in the composition of the CA were shown, their dynamics during the bacterial growth and their total concentration in CL were strain specific characteristic. The differences in the composition and dynamics of the CA, which are specific only to probiotic and opportunistic strains were revealed
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INTRODUCTION OF SOME RARE AND ENDANGERED SPECIES OF WESTERN CAUCASUS FLORA TO IN VITRO CULTURE
DescriptionThere were developed some methods of sterilization and introduction of the following endangered species of Caucasian flora to in vitro culture: Campanula sclerophylla Kolak., Lilium caucasicum Miscz. ex Grossh., Daphne woronowii Kolak., Pancratium maritimum L.. The effect of different plant hormones as well as their combinations and concentrations on the regeneration, growth and development of plants is studied. As a result of clonal propagation, more than 100 plants of Campanula sclerophylla Kolak, about 30 plants of Pancratium maritimum L., and 150 samples of Lilium caucasicum Miscz. Ex Grossh are saved in tissue culture
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MORPHOLOGICAL AND CYTO-BIOCHEMICAL BASIS OF SHOOT FORMATION OF CEREALS
DescriptionProcess of shoot formation of cereals is associated with the formation of zone shortened internodes, establishes buds certain capacity and completes transition by growth and formation of crop aboveground mass of each individual. Process of shoot formation is divided into three phases - preparatory, hidden and visible tillering, each of which is different peculiar morphological and cytobiochemical development
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Description
The article is dedicated to the features of the correlations between the indicators of shoot and root growth ability of grape cuttings varieties of Moldova and Vostorg of different lengths, under the influence of the processing them with Norwegian growth regulator - Radix plus. We have found that in the control variants on both varieties there are fairly close correlation connections between the number of shoots, their length and the degree of blooming buds. However, in the advanced options the character of the relationship between these parameters depends on the varietal characteristics manifested in the potential root activity cuttings. For Moldova cuttings which are characterized by high root activity, the use of Radix plus led to some strengthening of the correlations, and for Vostorg (with the weak activity of rooting of cuttings) - to decreasing of the correlations. This fact may indicate that the negative effects of growth regulators on the activity of endogenous hormones in the cuttings of Vostorg varieties, which explains the decrease of rooting of longer cuttings. Processing the cuttings of Moldova with Radix plus led to increasing in the frequency and extension of the correlations between shoot and root growth ability of cuttings. For Vostorg, on the contrary, the use of Radix plus led to the weakening of these connections. The reason for this phenomenon, as is the case with figures of shoot growth ability may be a change in the activity of the endogenous phytohormone influenced by exogenous auxin. Character of correlations identified between indicators of root growth ability of cuttings shows that rooting depends primarily on the hormonal activity of cuttings, number of roots - on cuttings plastic substances, and the yield of cuttings with 3 roots and more - on both of these points