03.00.00 Biological sciences
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Description
The article presents the materials of the study of the features of cultivation of Lactobacillus sp. on different culture medium. We have made a comparison of standard culture medium for cultivation Lactobacillus sp. and determined the most optimal culture medium
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STRUCTURAL AND PHYLOGENETIC ANALYSIS OF VVMYBA1, VVMYBA2 AND VVMYBA3 GRAPEVINE GENES
DescriptionThe article presents the structural and phylogenetic analysis of VIT_02s0033g00410, VIT_02s0033g00390 and VIT_02s0033g00450 genes of the grapevine genome and the closely related orthologous gene MYB114 of the Arabidopsis genome. These genes are responsible for the biosynthesis of anthocyanin in the organs of model plants and are of interest not only for practical production and breeding, but also for fundamental research. These genes were analyzed for GCcomposition of nucleotides, the presence of cisregulatory elements and promoter regions. DNA and protein sequences were aligned to look for similar elements, which allowed further analysis of the ultraconservative domains of four genes. Based on the results of search and identification of the conservative regions, a cluster tree was constructed, which made it possible to identify the separation of gene sidelines from, presumably, the main one. At the same time, the construction of consensus trees based on DNA and protein sequences revealed their absolute similarity. "The Minimal Evolution Tree" allowed calculating the approximate dates of the appearance of the mutations and the divergence times of the gene branches between each other. At the same time, the appearance of the Vitis genus and its separation from the Rosales was taken as the time first divergence point. In the end, homologous metabolic pathways were searched between grapevine and Arabidopsis, which revealed the presence of homologous proteins in the grape proteome. In this turn, it already confirms the existence of similar biosynthetic pathways and, as a consequence, interactions such as "DNA-protein" and "protein-protein"
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MICROBIAL COMMUNITIES OF KARPOSPHAERA AND PHILLOPLAN OF SOME PLANTS OF GROSSULARIACEAE FAMILY
DescriptionThe article presents the results of the research of microflora composition of the leaf surface, the surface of fruit and flowers of Ribes nigrum, R. niveum, R . rubrum and Grossularia reclinata. The research was carried out in the seasonal dynamics from 2007 to 2014. The authors used the method of print for isolating microorganisms from plants. For convenience of calculations and contamination comparison of different environmental niches of plants the number of selected microorganisms was counted on the surface of 1 cm2. It was found out in the article that microorganisms on the surface of lamina are distributed unequally. The number of microorganisms on the bottom surface of the foliage in all periods turned out to be higher. The greatest number of microorganisms was recorded in autumn and reaches the highest value in October. The number of microorganisms on fruit surface increased with ripening and was the highest in July. The quantity on the flowers varies considerably throughout the flowering and is represented minimally compared to other plants niches. The study revealed species-specificity of microorganisms and the host plants. At the same time, long-term study of the microflora of plants belonging to one family made it possible to reveal species that are typical epiphytes
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THE VARIABILITY OF TAXUS BACCATA CYPRESS CONES AT POPULATION OF DAGESTAN
DescriptionIn the populations of Taxus baccata of Foothills and Inside Mountain Dagestan, we have studied intra- and interpopulation variability of morphological characters of yew berry. The article has a contribution of interpopulation differences in the feature of "diameter of funnel" with the highest rates in Buynaksk population and dependency of the form of yew berry from this feature. According to the results of discriminant analysis, we have revealed a distinction in populations on this basis, although the classification matrix has not given 100% of the forecast for any of them. It is found, that the seeds of Khunzah population have relatively large dimensions and yew berry has more flattened shape compared to piedmont populations, while the differences in populations based on seeds are more pronounced than based on yew berry at low volatility of the characteristics of the first indicators. It is shown, that the characteristics of seeds and yew berry of Khunzakh population indicate the presence of certain genetic differences between populations inside mountain and foothills, and that the higher dissemination activity of first population is aimed at self-preservation, due to the less favorable conditions for the species. It is expected that the low variability of the average values for yew berries of cypress characteristics indicates the stability of the soil and climatic conditions of its habitats
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FEATURES OF PLANT INTRODUCTION IN THE KUBAN REGION
DescriptionThe features of introduction of trees, shrubs and herbaceous species in terms of the Krasnodar region and the south of the CIS are analyzed, the specificity of this process for deciduous and coniferous species, and grasses at their development in the spring and autumn periods, the allocation of forming niches generative organs, microsporogenesis, etc. are discussed. Opportunity to adapt different kinds of herbs and species of shrubs and trees, taking into account seasonal development with allocation of exotic species in the annual cycle of vegetation critical periods for the growing season Kuban has been considered. The results of the horizontal and vertical introduction in the study of the imported seeds and planting material of different species of trees, shrubs and grasses are discussed
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PAINTINGS IN TEACHING THE DISCIPLINE OF "GENETIC MONITORING"
DescriptionThe article deals with the use of paintings as illustrative and analytical material in the course of "Genetic monitoring", like sections of "Human Genetics", "Epigenetics". Using the paintings of great artists as the visual material in the learning process performs several tasks: analytical, informational and resourcefu
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INTERACTIONS OF GOLD, SILVER AND MAGNESIUM NANOPARTICLES WITH PLANTS
DescriptionThe review examines the recent years’ data pertaining to the interaction of plants with nanoparticles of noble metals (gold and silver) and with nanoparticles of magnesium as an element that has a central role in the organization of the cellular protein-synthesizing apparatus. Information is presented about the effect of gold and silver nanoparticles on plants and on possible nanoparticle phytotoxicity. It is shown that magnesium nanoparticles, as well as those of noble metals, produce various effects on the growth and productivity of plants, enabling the arsenal of biologically active substances to be expanded. Analysis is made of the involvement of magnesium nanoparticles in cryobiological reaction of the regular breakdown of plant RNA under conditions of ultra low temperatures, which is important not only for the further developments of views of the origin of life ob Earth but also for the purposes of designing new markers for the analysis of the biological peculiarities of agricultural crops on the basis of studies of magnesium in the long-lived RNA of their mature seeds. The presented critical analysis of the literature data, which are often incomplete and contradictory, suggest that a coordinated research program is needed that would detect correlation between particle parameters, experimental design, and observed biological effects
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Description
The creation of neotropical seashore plants exposition in the New conservatory is described. Data on floristic and ecological aspects of this plant formation are given. General description of species, presented in exposition is provided. An accelerated dynamics of plants development in new conditions is specified for the latest five years. Recommendations for neotropical seashore plants cultivation are given
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INFLUENCE OF THE BIOGEOHORIZONS ON THE CONDITION OF OAK PLANTINGS
DescriptionThe article reflects the influences of the biogeohorizons on structure of oak plantings. It also shows biomass model of oak trees and its satellites in the studied areas
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DYNAMICS OF RESTORATION OF BIOLOGICAL PROPERTIES OF BLACK SOILS POLLUTED WITH OIL
DescriptionNegative impact of oil on biological properties of soils right after pollution is shown in the article. Eventually, there is their restoration. However, even in some years after pollution, the biological properties of soils aren't restored completely