03.00.00 Biological sciences
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SPECIFICITY OF TRILON B INFLUENCE ON THE GERMINATION OF SEEDS OF VARIETIES OF WINTER BARLEY
DescriptionThe problem of increasing resistance of winter barley to adverse conditions of winter is the most problem in the selection area of this crop. The main component of this complex trait is the frost resistance. Laboratory methods of assessment of frost resistance are important for breeding of winter barley. These methods can effectively replace more costly method of direct freezing in the freezers. Magnesium cations (Mg++) are essential components of the protein-synthesizing system of germinating seeds. Magnesium cations determine the stability and translational activity of mRNA and rRNA. Trilon B is usually used for in vitro banding of magnesium cations in area of biochemistry of the nucleic acids. It was very interesting to verify the Trilon B influence to seed germination of winter barley varieties by experiment. Experiments showed: actinomycin D – the inhibitor of RNA synthesis – didn’t show varieties specific effect to seed growth of winter barley, while Trilon B had a varieties specific impact to length coleoptiles and roots. Research was carried out on etiolated seedlings at temperatures from 22 to 28oC, and different concentrations of Trilon B - 1,6×10-3М, 2,4×10-3М and 3,2×10-3М. The roots of 3-days old seedlings were more sensitive to Trilon B influence. It is shown the varieties of Russian origin were naturally reacted to Trilon B: the higher the frost resistance of variety, the more resistant variety to the action of various concentrations of Trilon B at different temperatures. The most optimum concentration was 3,2 × 10-3М at 22-25oC. Varieties by foreign origin deviated from this pattern. It is interesting to research the genetic essence of these differences. It will be helpful for optimization of varieties differentiation conditions to create a laboratory method of estimate of frost resistance of winter barley. The new method will be most efficient compared the direct freezing method
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Description
Along with traditional ways, the biotechnological methods become more significant in contribution to plant conservation in situ. The work is dedicated to elaboration and advancement of the method of isolated tissues and organs culture for conservation and reproduction of the badan, Bergenia crassifolia L., the rare species of Saxifrgaceae family. The conducted study yielded the regenerated plants of B. crassifolia from seeds. For the first time, we have performed conservation and reproduction of B. crassifolia in vitro under conditions of Yakutia
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INTRODUCTION OF IRIDACEAE IN CENTRAL YAKUTIA
DescriptionThe results of introduction test of species and cultivars variety of irises in the Central Yakutia are described in this article. Climatic conditions of the introduction region are extreme and they are strong restrictive factor at introduction to the culture of many other species and cultivar of decorative plants. The condition of plants after rewintering was estimated on a 4-mark scale. Total number of the plants which were lost in the first winter (0 points) is 126 species and cultivars that is 60,9%. For 01.06.2015 year a collection of irises in Yakutia makes 40 exemplar of species (22 species) and 31 exemplar of cultivars (30 cultivars), 7 species of them are successfully adapted (3 points) (Iris biglumis Vahl., I. bloudowii Ledeb., I. potaninii Maxim., I. scariosa Willd., I. setosa Pallas ex Link., I. tigridia Bunge, Pardanthopsis dichotoma (Pallas) Lenz). The listed species annually plentifully blossom, form seeds and intensive form escapes. Four species Iris biglumis, I. scariosa, I. bloudowii, Pardanthopsis dichotoma are form stable self-seedling
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Description
In the article we have stated results of the monitoring of local populations of rare plans species in the territory of the nature monument (NM) of the Rostov region «Raznotravno-tipchakovo-kovylnaya steppe» (Zernogradsky district). Vegetation of NM belongs to the poor version of the Azov steppe, flora contains 291 species, including 13 rare species (Astragalus ponticus Pall., Bellevalia sarmatica (Pall. ex Georgi) Woronow, Calophaca wolgarica (L. f.) DC., Caragana scythica (Kom.) Pojark., Centaurea talievii Kleop., Crambe pinnatifida R. Br., C. tataria Sebeok, Crocus reticulatus Stev. ex Adam, Eriosуnaphe longifolia (Fisch. ex Spreng.) DC., Iris pumila L., Stipa pulcherrima К. Koch, S. ucrainica P. Smirn., Tulipa schrenkii Regel), included in the Red List of the Rostov region, including 6 species included in the Red List of the Russian Federation. In 2016 were specified the patterns of rare species distribution in NM borders, and also other important parameters of their populations (number, density, age structure, etc.). The group of rare plants species, included in the Red List of the Rostov region, in the territory of NM includes 65 % of their total number known in Zernogradsky district. The special sozological value of NM is determined by the large local populations of Calophaca wolgarica, Astragalus ponticus and Crambe pinnatifida. Local populations of all rare species in the territory of NM can be considered to be steady as inhabit here during last 50 years
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"RARE PUMPKIN" IN STAMPS – THE HISTORY OF PLANTS, DISTRIBUTION AND ICONOGRAPHY
DescriptionIn the article we consider illustrative images of rare pumpkin cultures presented in stamps of different countries. In this work, we analyze stamps which represent rare pumpkin cultures. Stamps are reflection of the economy; they portray agriculture and elements of technology of agricultural production. Cucurbitaceae family includes a large number of species that are very different from each other in the form of fruits, in purpose, in origin. The objective of our study was to conduct a visual analysis of rare pumpkin crops, which are used in the world; to reflect the research progress on the introduction and the achievement of breeding work. The article provides an analysis of several cultures: momordika (Momordica charantia L.), Kiva (Kiwano), horned melon, cucumber Antilles, (Cucumis metuliferus L.); Akantositsios Naudin (Acanthosicyos naudinianus L.); momordica balsamina (Momordica Balsamina L.); koktsiniya (Coccinia sessifolia L.); luffa cylindrical (Luffa cylindrica L.); Lagenaria (Lagenaria siceraria (Molina) Standl.); cucumber snake (Trichosanthes anguina L.); antilles cucumber, anguria (Cucumis anguria L.); african cucumber (Cucumis africanus Lindl); wax gourd (Benincasa hispida (Thunb) Cogn..); chayota or mexican cucumber (Sechium edule Swartz). The analysis of the iconography of images of rare pumpkin crops in stamps of different countries allowed us to see the history of culture introduction of wild pumpkin from the local and foreign flora
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Description
Microsatellite DNA markers are currently used effectively in the study of the genetic diversity of the gene pool of fruit crops and DNA certification of varieties. For plum now there is rather limited list of works on the development of this type of DNA markers. Most often, the search for new SSR-markers for this species is carried out by checking of crossreproducibility of SSR-markers developed in other species of the genus Prunus. In this study, for the 18 SSR-markers previously developed on a peach, there was performed testing and evaluation of the prospects for the use of the genotyping of plum cultivars. Testing was made on the 4 varieties of genetically distant, belonging to the 4 different subspecies of Prunus domestica L., showed the effectiveness of their use. During the study, all tested DNA-markers were grouped together in multiplex sets comprising 3-4 markers. This allows simultaneous genotyping of 3-4 loci in a single PCR reaction. These multiplex kits are available for use in the study of genetic polymorphism of species Prunus domestica L
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Description
The article presents materials about selection and optimization of the nutrient medium for cultivation of Propionibacterium shermanii. We have shown a high positive effect expressed in the intensive growth of microorganisms of Propionibacterium shermanii on optimized medium with the juice from the tomatoes, as well as replacement of glucose on corn extract, which allows concluding that these components have a positive impact on the accumulation of biomass of Propionibacterium shermanii
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MICROORGANISMS OF PEDOSPHERE AND PECULIARITIES OF THE SOIL COVER OF AGRICULTURAL LANDSCAPES
DescriptionThe soil by it physical and chemical properties is a poly-dispersed heterogeneous multicomponent unique environment for development of most microorganisms. The soil is the richest natural substrate according to microbial gene fund. Presence of plants and animals in soil maintains it heterogeneous as environment of soil microorganisms, which are main regulators of natural gas composition of atmosphere of Earth, including its macro- and micro-components (including the main "greenhouse" gases - methane, carbon dioxide, nitrous oxide). Recently, the elucidation of these singularities of vital activity of soil organisms has resulted in general conclusion - due to them soil cover serves as a global bio-geo-chemical membrane, through which the exchange of matter and energy between pedosphere, lithosphere, atmosphere, hydrosphere and main living inhabitants of Earth does its work
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THE APPLICATION OF THE BACTERIOPHAGES TO COMBAT COLIBACILLOSIS AND CAMPYLOBACTERIOSIS IN POULTRY
DescriptionThis work considers the needs and methods of phage therapy of two bacterial infections in poultry. The review examines the use of bacteriophages, from the moment of their discovery to the present day. We discuss the relevance of such diseases as compilobacteriosis and colibacteriosis transmitted from birds to humans, we show how to use bacteriophages and their efficacy in models of adult birds and chickens, including information provided on the pilot study of phage therapy at a poultry farm. The reported study was partially supported by RFBR, research projects No. 16-44-230855 and No. 13-04- 00991 and № 16-44-230855-р_а
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DEVELOPMENT OF A FUNCTIONAL FEED ADDITIVE
DescriptionThe new feed additives for animals based on milk whey, enriched with sprouting wheat, barley, maize grains and lactic-acid bacterium have been presented in this article. This study explores the possibility of combining the prebiotics ability of milk whey and sprouting grains with feed probiotic attributes of microbe to enhance gut health of animals and digestibility of the feed. Twelve variants of products were tested in the study using the microbial and physical-chemical approaches. All the assays showed high count of microorganism and high content of reducing sugar. The results of investigation indicate that selected feed additive show high quality. An additional point is that it contains useful organic acids (lactic, acetic and propionic acids) and 2,1×109 colony-forming unit of probiotics microorganism that hold the concentration for 4 months. There are not yeast, must, coliform bacteria and Staphylococcus aureus in the developed functional feed product. It contributes to the normalization of the microflora of the gastrointestinal tract of animal, suppression of conditionally pathogenic and putrefactive microflora. The elaborated feed component will help to provide combined feed companies with accessible high-quality raw material